通过流体力学方法对荚膜多糖及其糖缀合物进行表征

Characterization of Capsular Polysaccharides and Their Glycoconjugates by Hydrodynamic Methods.

作者信息

Harding Stephen E, Abdelhameed Ali Saber, Gillis Richard B, Morris Gordon A, Adams Gary G

机构信息

National Centre for Macromolecular Hydrodynamics, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, LE12 5RD, UK,

出版信息

Methods Mol Biol. 2015;1331:211-27. doi: 10.1007/978-1-4939-2874-3_13.

DOI:10.1007/978-1-4939-2874-3_13

PMID:26169743

Abstract

Hydrodynamic methods are relevant for the characterization of carbohydrates such as capsular bacterial polysaccharides or glycoconjugates in solution. This chapter focuses on the following hydrodynamic methods: sedimentation velocity analytical ultracentrifugation (SV AUC), dynamic light scattering (DLS), sedimentation equilibrium analytical ultracentrifugation (SE AUC), size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS), and capillary viscometry-intrinsic viscosity measurement. The chapter highlights the general principle of these five methods, describes experimental details, and specifies advances in the last years.

摘要

流体动力学方法适用于表征溶液中的碳水化合物，如荚膜细菌多糖或糖缀合物。本章重点介绍以下流体动力学方法：沉降速度分析超离心法（SV AUC）、动态光散射法（DLS）、沉降平衡分析超离心法（SE AUC）、尺寸排阻色谱-多角度光散射联用技术（SEC-MALS）以及毛细管粘度测定-特性粘度测量。本章突出了这五种方法的一般原理，描述了实验细节，并详述了近年来的进展。