National Centre for Macromolecular Hydrodynamics, University of Nottingham, Sutton Bonington LE12 5RD, UK.
National Centre for Macromolecular Hydrodynamics, University of Nottingham, Sutton Bonington LE12 5RD, UK; Faculty of Medicine and Health Sciences, University of Nottingham, Clifton Boulevard, Nottingham NG9 2RD, UK.
Carbohydr Polym. 2012 Sep 1;90(1):237-42. doi: 10.1016/j.carbpol.2012.05.030. Epub 2012 May 15.
Capsular polysaccharides from ten different serotypes of Streptococcus pneumoniae have been studied with regards their hydrodynamic properties in solution, namely their sedimentation coefficient and molar mass distributions, solution conformations and flexibilities (persistence lengths Lp), important properties for the construction of polysaccharide and glycoconjugate vaccines. Sedimentation and molar mass distributions (obtained by sedimentation velocity and equilibrium analysis in the analytical ultracentrifuge supported by size exclusion chromatography coupled to multi-angle light scattering measurements) were generally unimodal, with weight (mass) average molar masses ranging from 100×10(3) to 1300×10(3) g/mol. Estimates of chain flexibilities from three different procedures applied to intrinsic viscosity, sedimentation coefficient and molar mass data, showed that the polysaccharides from all the serotypes studied had semi-flexible structures in solution with persistence lengths in the range from ∼4 to 9 nm.
已研究了来自 10 种不同血清型肺炎链球菌的荚膜多糖，以研究其在溶液中的流体力学性质，即沉降系数和摩尔质量分布、溶液构象和柔韧性（持久长度 Lp），这些性质对于多糖和糖缀合物疫苗的构建很重要。沉降和摩尔质量分布（通过分析超速离心中的沉降速度和平衡分析以及尺寸排阻色谱与多角度光散射测量相结合获得）通常是单峰的，重均（质量）平均摩尔质量范围为 100×10^3 至 1300×10^3 g/mol。应用于特性粘度、沉降系数和摩尔质量数据的三种不同程序估算的链柔韧性表明，在所研究的所有血清型的多糖在溶液中均具有半柔性结构，持久长度在 4 至 9 nm 范围内。
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