Schlaen Rubén Gustavo, Mancini Estefanía, Sanchez Sabrina Elena, Perez-Santángelo Soledad, Rugnone Matías L, Simpson Craig G, Brown John W S, Zhang Xu, Chernomoretz Ariel, Yanovsky Marcelo J
Fundación Instituto Leloir, Instituto de Investigaciones Bioquímicas de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas, C1405BWE, Buenos Aires, Argentina;
Cell and Molecular Sciences, James Hutton Institute, Invergowrie, Dundee DD2 5DA, United Kingdom;
Proc Natl Acad Sci U S A. 2015 Jul 28;112(30):9382-7. doi: 10.1073/pnas.1504541112. Epub 2015 Jul 13.
The mechanisms by which poikilothermic organisms ensure that biological processes are robust to temperature changes are largely unknown. Temperature compensation, the ability of circadian rhythms to maintain a relatively constant period over the broad range of temperatures resulting from seasonal fluctuations in environmental conditions, is a defining property of circadian networks. Temperature affects the alternative splicing (AS) of several clock genes in fungi, plants, and flies, but the splicing factors that modulate these effects to ensure clock accuracy throughout the year remain to be identified. Here we show that GEMIN2, a spliceosomal small nuclear ribonucleoprotein assembly factor conserved from yeast to humans, modulates low temperature effects on a large subset of pre-mRNA splicing events. In particular, GEMIN2 controls the AS of several clock genes and attenuates the effects of temperature on the circadian period in Arabidopsis thaliana. We conclude that GEMIN2 is a key component of a posttranscriptional regulatory mechanism that ensures the appropriate acclimation of plants to daily and seasonal changes in temperature conditions.
变温生物确保生物过程对温度变化具有稳健性的机制在很大程度上尚不清楚。温度补偿是昼夜节律网络的一个决定性特性，即昼夜节律在环境条件季节性波动导致的广泛温度范围内维持相对恒定周期的能力。温度会影响真菌、植物和果蝇中几个时钟基因的可变剪接（AS），但调节这些效应以确保全年时钟准确性的剪接因子仍有待确定。在这里，我们表明，GEMIN2是一种从酵母到人类都保守的剪接体小核糖核蛋白组装因子，它调节低温对大量前体mRNA剪接事件的影响。特别是，GEMIN2控制几个时钟基因的可变剪接，并减弱温度对拟南芥昼夜周期的影响。我们得出结论，GEMIN2是一种转录后调控机制的关键组成部分，该机制可确保植物适当地适应温度条件的每日和季节性变化。
