胃泌素释放肽在卵巢癌ES2细胞中的意义。
Significance of gastrin-releasing peptide in ovarian cancer ES2 cells.
作者信息
Jia Yanyan, Shi Huirong, Fan Dongmei
机构信息
Department of Gynecology, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan 450052, P.R. China.
Department of Gynecology, The First Affiliated Hospital, Henan Scientific and Technologic University, Luoyang, Henan 471003, P.R. China.
出版信息
Oncol Lett. 2015 Jul;10(1):359-363. doi: 10.3892/ol.2015.3240. Epub 2015 May 20.
The present study aimed to investigate the effect of gastrin-releasing peptide (GRP) on the proliferation and invasion of ovarian cancer ES2 cells. The ovarian cancer ES2 cells were transfected with small interfering RNA against GRP. Cell proliferation was assessed using the Trypan blue assay, apoptosis was determined using propidium iodide/fluorescein isothiocyanate and flow cytometry, and the invasion ability was detected using the Transwell assay. The results revealed that the expression of GRP significantly decreased following transfection with GRP-short hairpin RNA. Furthermore, the silencing of GRP resulted in increased apoptosis and a reduced invasive ability of the ES2 cells. It was concluded that GRP may regulate the proliferation and migration of human ovarian cancer cells, which indicates that GRP may be a potential novel target for the treatment of ovarian cancer.
本研究旨在探讨胃泌素释放肽（GRP）对卵巢癌ES2细胞增殖和侵袭的影响。用针对GRP的小干扰RNA转染卵巢癌ES2细胞。采用台盼蓝试验评估细胞增殖，用碘化丙啶/异硫氰酸荧光素和流式细胞术测定细胞凋亡，并用Transwell试验检测侵袭能力。结果显示，用GRP短发夹RNA转染后，GRP的表达显著降低。此外，GRP的沉默导致ES2细胞凋亡增加和侵袭能力降低。得出的结论是，GRP可能调节人卵巢癌细胞的增殖和迁移，这表明GRP可能是治疗卵巢癌的潜在新靶点。
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