Bai Yaling, Zhang Junxia, Xu Jinsheng, Cui Liwen, Zhang Huiran, Zhang Shenglei, Feng Xunwei
Department of Nephrology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China.
Biomed Rep. 2015 Jul;3(4):593-597. doi: 10.3892/br.2015.473. Epub 2015 May 27.
Vascular calcification (VC), in which high serum phosphate plays a critical role, is one major problem in patients with chronic kidney disease. Clinical studies report that magnesium has a protective effect on VC. However, the studies regarding the impact of high serum magnesium on VC at a cellular level are few and require further investigation. Therefore, the present study explored the effect of magnesium on calcification induced by β-glycerophosphate (BGP) in rat aortic vascular smooth muscle cells (RAVSMCs). In the present study, the addition of magnesium decreased calcium deposition, which was increased by BGP. Higher magnesium levels inhibited BGP-induced alkaline phosphatase (ALP) activity and decreased the expression of core-binding factor α-1 (Cbfα1). In conclusion, higher magnesium levels prevented BGP-induced calcification in RAVSMCs and inhibited the expression of Cbfα1 and ALP. Thus, magnesium is influencing the expression of Cbfα1 and ALP associated with VC and may have the potential to serve as a role for VC in clinical situations.
血管钙化(VC)是慢性肾病患者面临的一个主要问题,其中高血清磷起着关键作用。临床研究报告称,镁对血管钙化具有保护作用。然而,关于高血清镁在细胞水平对血管钙化影响的研究较少,需要进一步探究。因此,本研究探讨了镁对β-甘油磷酸(BGP)诱导的大鼠主动脉血管平滑肌细胞(RAVSMCs)钙化的影响。在本研究中,添加镁减少了由BGP增加的钙沉积。较高的镁水平抑制了BGP诱导的碱性磷酸酶(ALP)活性,并降低了核心结合因子α-1(Cbfα1)的表达。总之,较高的镁水平可预防BGP诱导的RAVSMCs钙化,并抑制Cbfα1和ALP的表达。因此,镁正在影响与血管钙化相关的Cbfα1和ALP的表达,并且在临床情况下可能对血管钙化具有潜在作用。