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提高公猪精子体外和体内受精能力的方法。

Methods for Improving In Vitro and In Vivo Boar Sperm Fertility.

作者信息

Funahashi H

机构信息

Department of Animal Science, Okayama University, Okayama, Japan.

出版信息

Reprod Domest Anim. 2015 Jul;50 Suppl 2:40-7. doi: 10.1111/rda.12568.

Abstract

Fertility of boar spermatozoa is changed after ejaculation in vivo and in vitro. During processing for in vitro fertilization (IVF), although spermatozoa are induced capacitation, resulting in a high penetration rate, persistent obstacle of polyspermic penetration is still observed with a high incidence. For artificial insemination (AI), we still need a large number of spermatozoa and lose a majority of those in the female reproductive tract. Fertility of cryopreserved boar spermatozoa is still injured through freezing and thawing process. In the present brief review, factors affecting fertility of boar sperm during IVF, AI and cryopreservation are discussed in the context of discovering methodologies to improve it.

摘要

公猪精子在体内和体外射精后,其生育能力会发生变化。在体外受精(IVF)处理过程中,尽管精子会被诱导发生获能,从而导致较高的穿透率,但仍会频繁观察到多精入卵穿透这一持续存在的障碍,且发生率很高。对于人工授精(AI)而言,我们仍然需要大量精子,并且在雌性生殖道中会损失大部分精子。冷冻保存的公猪精子的生育能力在冷冻和解冻过程中仍然会受到损害。在本简要综述中,将在探索提高公猪精子生育能力方法的背景下,讨论影响公猪精子在体外受精、人工授精和冷冻保存过程中生育能力的因素。

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