开发一种快速、可靠且定量的方法——“SPOTi”用于测试抗真菌功效。
Development of a rapid, reliable and quantitative method--"SPOTi" for testing antifungal efficacy.
作者信息
Rizi Khalida, Murdan Sudaxshina, Danquah Cynthia A, Faull Jane, Bhakta Sanjib
机构信息
Department of Pharmaceutics, UCL School of Pharmacy, 29-39 Brunswick Square, London WC1N 1AX, UK; Department of Biological Sciences, Institute of Structural and Molecular Biology, Birkbeck, University of London, Malet Street, London WC1E 7HX, UK.
Department of Pharmaceutics, UCL School of Pharmacy, 29-39 Brunswick Square, London WC1N 1AX, UK.
出版信息
J Microbiol Methods. 2015 Oct;117:36-40. doi: 10.1016/j.mimet.2015.07.005. Epub 2015 Jul 13.
A reference method for the antimicrobial susceptibility testing of common fungal pathogens such as dermatophytes, is currently lacking. In this study, we report the successful adaptation of solid agar-based spot culture growth inhibition assay (SPOTi) for dermatophytes, currently being used as a gold-standard in the anti-tubercular drug discovery field. The fungal-SPOTi assay correlated with the disc-diffusion method, and is validated using mycelial plugs. We propose the fungal-SPOTi as a high-throughput alternative to the disc-diffusion and broth micro-dilution anti-fungal assays to screen novel anti-fungals.
目前缺乏针对皮肤癣菌等常见真菌病原体进行抗菌药敏试验的参考方法。在本研究中，我们报告了基于固体琼脂的斑点培养生长抑制试验（SPOTi）成功适用于皮肤癣菌，该试验目前在抗结核药物发现领域被用作金标准。真菌SPOTi试验与纸片扩散法相关，并使用菌丝块进行了验证。我们提出将真菌SPOTi作为纸片扩散法和肉汤微量稀释抗真菌试验的高通量替代方法，用于筛选新型抗真菌药物。
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