小龙虾（）视网膜中的类视黄醇代谢以及视黄醇向脱氢视黄醇的转化
RETINOID METABOLISM AND CONVERSION OF RETINOL TO DEHYDRORETINOL IN THE CRAYFISH () RETINA.
作者信息
Suzsuki Tatsuo, Terakita Akihisa, Tsin Andrew T C
机构信息
Department of Pharmacology, Hyogo College of Medicine, Hyogo 663, Japan.
Photodynamics Research Center, Institute of Physical and Chemical Research, Miyagi 981, Japan.
出版信息
Comp Biochem Physiol B. 1993 Jun;105(2):257-261. doi: 10.1016/0305-0491(93)90226-U.
Activities of retinylester hydrolase (REHase) and retinol dehydrogenase (RDHase) were detected in the crayfish retina which has two visual pigment chromophores and three stored retinoids.RDHase and 11- REHase activities were localized to rhabdomal fraction and enzymes were specific to 11- isomers of substrates. Overall reaction from retinylester to retinal was highly specific to 11- isomers and this reaction was not selective between retinol and dehydroretinol.When 11- [H]-retinol was incubated with retina homogenates, radioactivity was detected in 11- dehydroretinol.These results suggest that retinol is converted to dehydroretinol after hydrolysis of retinylesters.
在具有两种视觉色素发色团和三种储存类视黄醇的小龙虾视网膜中检测到了视黄酯水解酶（REHase）和视黄醇脱氢酶（RDHase）的活性。RDHase和11 - REHase活性定位于视杆部分，且这些酶对底物的11 - 异构体具有特异性。从视黄酯到视黄醛的整体反应对11 - 异构体具有高度特异性，并且该反应在视黄醇和脱氢视黄醇之间没有选择性。当将11 - [H] - 视黄醇与视网膜匀浆一起孵育时，在11 - 脱氢视黄醇中检测到放射性。这些结果表明，视黄酯水解后视黄醇会转化为脱氢视黄醇。
Zotero 插件