在开始抗逆转录病毒治疗的幼儿中使用干血斑标本进行HIV-1耐药基因分型。
The use of dried blood spot specimens for HIV-1 drug resistance genotyping in young children initiating antiretroviral therapy.
作者信息
Salimo Anna T, Ledwaba Johanna, Coovadia Ashraf, Abrams Elaine J, Technau Karl-Günter, Kuhn Louise, Morris Lynn, Hunt Gillian M
机构信息
Centre for HIV and STIs, National Institute for Communicable Diseases, National Health Laboratory Services, Johannesburg, South Africa.
Empilweni Services and Research Unit, Rahima Moosa Mother and Child Hospital, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.
出版信息
J Virol Methods. 2015 Oct;223:30-2. doi: 10.1016/j.jviromet.2015.07.005. Epub 2015 Jul 17.
Paired plasma and dried blood spots (DBS) from 232 South African HIV-infected children initiating antiretroviral therapy (ART) were genotyped for drug resistance mutations, most of who had prior exposure to ART for prevention-of-mother-to-child-transmission. Non-nucleoside reverse transcriptase inhibitor mutations were most commonly detected in both specimen types, particularly Y181C/I and K103N/S. Resistance interpretation concordance was achieved in 97% of pairs with seven children having mutations detected in DBS only. These results validate the preferential use of DBS specimens for HIVDR genotyping in this patient group.
对232名开始接受抗逆转录病毒治疗（ART）的南非感染艾滋病毒儿童的配对血浆和干血斑（DBS）进行耐药性突变基因分型，其中大多数儿童此前曾接受过预防母婴传播的ART治疗。两种样本类型中最常检测到非核苷类逆转录酶抑制剂突变，尤其是Y181C/I和K103N/S。97%的配对实现了耐药性解读一致性，7名儿童仅在DBS中检测到突变。这些结果证实了在该患者群体中优先使用DBS样本进行HIVDR基因分型。
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