Irum Shamaila, Ahmed Haroon, Mukhtar Muhammad, Mushtaq Muhammad, Mirza Bushra, Donskow-Łysoniewska Katarzyna, Qayyum Mazhar, Simsek Sami
Department of Biosciences, COMSATS Institute of Information Technology (CIIT), Islamabad, Pakistan; Department of Zoology and Biology, PMAS-Arid Agriculture University, Rawalpindi, Pakistan.
Department of Biosciences, COMSATS Institute of Information Technology (CIIT), Islamabad, Pakistan.
Vet Parasitol. 2015 Sep 15;212(3-4):451-5. doi: 10.1016/j.vetpar.2015.06.028. Epub 2015 Jul 2.
Current study was designed to evaluate in vivo and in vitro anthelmintic activity of Artemisia vestita Wall ex DC. and Artemisia maritima L. against Haemonchus contortus in comparison with ivermectin to investigate the effect of plant extracts on survival of infective L3 and adults under in vitro condition. Plant extracts were given to H. contortus infected sheep orally and it was infected with L3 stage of H. contortus at dose of 5000 larvae/sheep. Total of 25-30 larvae were incubated with plant extracts in PBS alone and ivermectin at different concentration used as positive control. It was recorded that there is a significant decrease in fecal egg count (FEC) after post-treatment period for both plants. The highest fecal egg count reduction for A. vestita was 87.2% at 100mg/kg while for A. maritima it was 84.5% on day 28 post-treatment. Investigated extracts indicated significant activity against larvae and adult worms.
本研究旨在评估由DC的Wall所描述的岩蒿（Artemisia vestita）和滨海蒿（Artemisia maritima L.）对捻转血矛线虫（Haemonchus contortus）的体内和体外驱虫活性，并与伊维菌素进行比较，以研究植物提取物在体外条件下对感染性L3幼虫和成虫存活的影响。将植物提取物口服给予感染捻转血矛线虫的绵羊，并以5000只幼虫/绵羊的剂量感染捻转血矛线虫的L3期。总共25-30只幼虫在仅含植物提取物的PBS中孵育，不同浓度的伊维菌素用作阳性对照。记录到两种植物在治疗后粪便虫卵计数（FEC）均显著下降。治疗后第28天，岩蒿在100mg/kg时粪便虫卵计数降低率最高，为87.2%，而滨海蒿为84.5%。所研究的提取物对幼虫和成虫均显示出显著活性。
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