在特级初榨橄榄油生物活性成分油橄榄苦素的细胞相互作用组中。

In Cell Interactome of Oleocanthal, an Extra Virgin Olive Oil Bioactive Component.

作者信息

Cassiano Chiara, Casapullo Agostino, Tosco Alessandra, Monti Maria Chiara, Riccio Raffaele

出版信息

Nat Prod Commun. 2015 Jun;10(6):1013-6.

PMID:26197539

Abstract

A copper-(I)-catalyzed variation of the Huisgen 1,3-dipolar cycloaddition has been applied to lead the in living-cell mass-spectrometry based identification of protein targets of oleocanthal, a natural metabolite daily ingested by millions of people. Chemical proteomics revealed heat-shock proteins, HSP70 and HSP90, as main oleocanthal interactors in living systems. These two proteins are involved in cancer development and, thus, our findings could have important outcomes for a deep evaluation of the bio-pharmacological significance of oleocanthal.

摘要

铜（I）催化的Huisgen 1,3-偶极环加成反应变体已被用于通过基于活细胞质谱法来鉴定油橄榄苦素的蛋白质靶点，油橄榄苦素是数百万人日常摄入的一种天然代谢产物。化学蛋白质组学揭示了热休克蛋白HSP70和HSP90是活细胞系统中油橄榄苦素的主要相互作用蛋白。这两种蛋白质都参与癌症发展，因此，我们的研究结果对于深入评估油橄榄苦素的生物药理学意义可能具有重要意义。