Li Weiwei, Huang Luli, Cai Weimei, Cao Sue, Yuan Yan, Lu Sheng, Zhao Yanzheng, Lu Ping
Department of Oncology, the 1 Affiliated Hospital of Xinxiang Medical University, Xinxiang Henan Prov., PR China.
Department of Cardiology, the 1 Affiliated Hospital of Xinxiang Medical University, Xinxiang Henan Prov., PR China.
Contemp Oncol (Pozn). 2015;19(1):17-21. doi: 10.5114/wo.2014.41391. Epub 2015 Mar 26.
To assess the potential association between serotonin transporter gene insertion/deletion polymorphism and the cancer-related constipation phenotype.
A total of 120 patients diagnosed with malignant solid tumors were subjected to genotyping. For the two groups - patients with constipation and constipation-free patients with non-gastrointestinal cancer, 60 cases in each group - we collected the peripheral venous blood. We extracted genomic DNA, and used polymerase chain reaction (PCR) to analyze the serotonin transporter (5-HT) link polymorphic region (5-HTTLPR) polymorphism of the serotonin transporter gene.
The frequency of S/S genotype in cancer patients with constipation was 66.67% (40/60), and the frequency of the S allele was 79.17% (95/120); the frequency of S/S genotype in cancer patients without constipation was 48.33% (29/60), and the frequency of the S allele was 65.83% (79/120). There was a significant difference between the two groups (p < 0.05).
The presence of 5-HTTLPRS/S genotype and the S allele in patients with cancers probably carry an increased risk of constipation. However, its role as a cause of cancer-related constipation needs to be further investigated.
评估血清素转运体基因插入/缺失多态性与癌症相关便秘表型之间的潜在关联。
对120例诊断为恶性实体瘤的患者进行基因分型。对于两组——便秘患者和无便秘的非胃肠道癌症患者，每组60例——我们采集外周静脉血。我们提取基因组DNA，并使用聚合酶链反应（PCR）分析血清素转运体基因的血清素转运体（5-HT）连锁多态性区域（5-HTTLPR）多态性。
便秘癌症患者中S/S基因型的频率为66.67%（40/60），S等位基因的频率为79.17%（95/120）；无便秘癌症患者中S/S基因型的频率为48.33%（29/60），S等位基因的频率为65.83%（79/120）。两组之间存在显著差异（p<0.05）。
癌症患者中5-HTTLPR S/S基因型和S等位基因的存在可能增加便秘风险。然而，其作为癌症相关便秘病因的作用需要进一步研究。
