Reversed phase high performance liquid chromatography of glycopeptides.
作者信息
Wang T H, Chen T, Barofsky D F
机构信息
Department of Agricultural Chemistry, Oregon State University, Corvallis 97331.
出版信息
Biomed Chromatogr. 1989 Nov;3(6):241-5. doi: 10.1002/bmc.1130030603.
PMID:2620144
Abstract
A practical procedure for isolating and purifying glycopeptides is described, viz. enzymatic hydrolysis-gel permeation chromatography-ion exchange chromatography-reversed phase HPLC. Using this procedure 28 glycopeptides from hen ovalbumin have been isolated some of which hitherto have not been identified. Water was a suitable mobile phase for preparing pure glycopeptides, and control of column temperature was important for good separations and reproducible retention times. Structural confirmation was by fast atom bombardment mass spectrometry.
摘要
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