Jones Alexander M
Department of Plant Biology, Carnegie Institution for Science, 260 Panama St, Stanford, CA, 94305, USA.
New Phytol. 2016 Apr;210(1):38-44. doi: 10.1111/nph.13552. Epub 2015 Jul 22.
Abscisic acid (ABA) is a key phytohormone promoting abiotic stress tolerance as well as developmental processes such as seed dormancy. A spatiotemporal map of ABA concentrations would greatly advance our understanding of the cell type and timing of ABA action. Organ and tissue-level ABA measurements, as well as indirect in vivo measurements such as cell-specific transcriptional analysis of ABA metabolic enzymes and ABA-responsive promoters, have all contributed to current views of the localization and timing of ABA accumulations. Recently developed Förster resonance energy transfer (FRET) biosensors for ABA that sense ABA levels directly promise to add unprecedented resolution to in vivo ABA spatiotemporal mapping and expand our knowledge of the mechanisms controlling ABA levels in space and time.
脱落酸(ABA)是一种关键的植物激素,它既能促进植物对非生物胁迫的耐受性,又能参与种子休眠等发育过程。ABA浓度的时空图谱将极大地推动我们对ABA作用的细胞类型和时间的理解。器官和组织水平的ABA测量,以及间接的体内测量,如对ABA代谢酶和ABA响应启动子的细胞特异性转录分析,都为目前关于ABA积累的定位和时间的观点做出了贡献。最近开发的用于直接检测ABA水平的基于荧光共振能量转移(FRET)的ABA生物传感器,有望为体内ABA时空图谱提供前所未有的分辨率,并扩展我们对控制ABA水平在空间和时间上的机制的认识。