Zhuang Xinyu, Zou Haitao, Shi Huoying, Shao Hongxia, Ye Jianqiang, Miao Ji, Wu Genghua, Qin Aijian
Ministry of Education Key Laboratory for Avian Preventive Medicine, Yangzhou University, No. 12 East Wenhui Road, Yangzhou, Jiangsu, 225009, P.R. China.
Key Laboratory of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, 225009, P.R. China.
BMC Vet Res. 2015 Jul 23;11:157. doi: 10.1186/s12917-015-0493-7.
Outbreaks of Marek's disease (MD), caused by Marek's disease virus (MDV), primarily occur in 10-12-week-old hens.
We report a case of MD in a breeding flock of 24-30-week-old vaccinated broilers in China. The clinical signs in the affected chickens appeared at 24 weeks, and the incidence of tumours peaked at 30 weeks. The morbidity and mortality of the hens were 5 % and 80 %, respectively. Hematoxylin-eosin staining of the tissues showed the typical characteristics of MD. MDV infection was confirmed in the hens with an agar gel diffusion precipitation assay for the MD antigen in the feather follicle epithelium. An MDV strain, designated AH1410, was isolated from the blood lymphocytes. Sequence analyses of the pp38, meq, and gB genes revealed that strain AH1410 had molecular features consistent with a virulent, previously identified MDV.
Our data provide evidence that not only is MDV becoming more virulent, but that the period of its onset in chickens is expanding. These findings provide the basis the molecular surveillance and further study of virulent MDV mutants and control strategies for MD in China.
由马立克氏病病毒（MDV）引起的马立克氏病（MD）疫情主要发生在10至12周龄的母鸡中。
我们报告了中国一群24至30周龄接种过疫苗的肉鸡种鸡群中发生MD的病例。受感染鸡的临床症状在24周时出现，肿瘤发病率在30周时达到峰值。母鸡的发病率和死亡率分别为5%和80%。组织苏木精-伊红染色显示出MD的典型特征。通过对毛囊上皮中MD抗原进行琼脂凝胶扩散沉淀试验，在母鸡中确认了MDV感染。从血液淋巴细胞中分离出一株MDV毒株，命名为AH1410。对pp38、meq和gB基因的序列分析表明，AH1410毒株具有与先前鉴定的强毒MDV一致的分子特征。
我们的数据表明，不仅MDV的毒性在增强，而且其在鸡群中的发病期也在延长。这些发现为中国强毒MDV突变体的分子监测、进一步研究以及MD的防控策略提供了依据。
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