使用细胞外通量分析仪对H9C2细胞进行生物能量学表征
BIOENERGETIC CHARACTERIZATION OF H9C2 CELLS USING THE EXTRACELLULAR FLUX ANALYZER.
作者信息
Scurtu Ileana, Sturza A, Pavel Ioanna Zinuca, Popescu Roxana, Privistirescu Andreea, Duicu Oana Maria, Muntean Danina Mirela
出版信息
Rev Med Chir Soc Med Nat Iasi. 2015 Apr-Jun;119(2):491-5.
UNLABELLED
The aim of the present work was to standardize the working methodology for assessing the bioenergetic profile of H9c2 cardiomyoblasts cells, with reference to the optimization of cell seeding number and the establishment of favorable concentrations for the classic modulators of mitochondrial respiratory function, in particular the one of a classical uncoupler, FCCP.
MATERIAL AND METHODS
The extracellular flux analyzer (XF, Seahorse Bioscience) is a novel high-throughput instrument able to monitor the metabolism of living cells by simultaneously measuring mitochondrial respiration and glycolysis. The in vitro platform will be further used to better understand the pathophysiology and the unrecognized side effects of drugs currently used in the therapy of major cardiovascular diseases.
CONCLUSIONS
In the long run, characterization of novel pharmacological agents' effects on other cell lines, including tumoral ones, will be also considered.
未标记
本研究的目的是规范评估H9c2心肌母细胞生物能量谱的工作方法，参考细胞接种数量的优化以及确定线粒体呼吸功能经典调节剂（特别是经典解偶联剂FCCP）的适宜浓度。
材料与方法
细胞外通量分析仪（XF，Seahorse Bioscience公司）是一种新型高通量仪器，能够通过同时测量线粒体呼吸和糖酵解来监测活细胞的代谢。该体外平台将进一步用于更好地理解目前用于治疗主要心血管疾病的药物的病理生理学和未被认识的副作用。
结论
从长远来看，还将考虑新型药物对其他细胞系（包括肿瘤细胞系）作用的表征。
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