采用细胞外通量分析仪对小鼠骨髓来源树突状细胞进行代谢分析。
Metabolic analysis of mouse bone-marrow-derived dendritic cells using an extracellular flux analyzer.
机构信息
Department of Clinical Chemistry and Laboratory Medicine, Graduate School of Medical Sciences, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
出版信息
STAR Protoc. 2021 Mar 26;2(2):100401. doi: 10.1016/j.xpro.2021.100401. eCollection 2021 Jun 18.
Dendritic cell (DC) maturation induced by Toll-like receptor (TLR) agonists requires the activation of downstream metabolic changes. Here, we provide a detailed protocol to measure glycolysis, mitochondrial respiration, and fatty acid oxidation in mouse bone-marrow-derived DCs with the Seahorse XF24 extracellular flux (XF) analyzer. XF analysis with the Seahorse bioanalyzer has become a standard method to measure bioenergetic functions in cells, and this protocol can be adapted to other immune cells. For complete information on using this protocol, please refer to Gotoh et al. (2018).
树突状细胞 (DC) 成熟受 Toll 样受体 (TLR) 激动剂诱导，需要激活下游代谢变化。在这里，我们提供了一种详细的方案，使用 Seahorse XF24 细胞外通量 (XF) 分析仪测量小鼠骨髓来源的 DC 的糖酵解、线粒体呼吸和脂肪酸氧化。XF 分析与 Seahorse 生物分析仪已成为测量细胞生物能量功能的标准方法，该方案可适用于其他免疫细胞。有关使用此方案的完整信息，请参阅 Gotoh 等人。(2018)。
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