Jones R T, Kreisberg J I, Linhardt G E, Trump B F
Adv Shock Res. 1978;1:197-207.
Exocrine pancreatic tissue from 65 male Sprague-Dawley rats was studied by light and electron microscopy to determine if acute lethal and sublethal alterations seen in the human exocrine pancreas following shock could be duplicated in an animal model. Two models were used: one in which 50% of the blood was withdrawn via cardiac puncture with no reinfusion and another in which the animal was subjected to a hypovolemic episode (40 mm Hg) for 60 minutes, with reinfusion of the blood. Animals were killed at various intervals, and pancreatic tissue was sampled for morphological study. No differences were seen between experimental and control animals by light microscopy. The main subcellular alteration seen using these models was the formation of numerous autophagic vacuoles. From these studies it appears that the alterations seen in the human pancreas in shock can be duplicated in the rat and that a shock model that involves removal of more than 50% of the animal's blood volume is necessary to cause irreversible cell damage to the exocrine pancreas.
通过光学显微镜和电子显微镜对65只雄性斯普拉格-道利大鼠的外分泌胰腺组织进行研究，以确定休克后人外分泌胰腺中出现的急性致死性和亚致死性改变是否能在动物模型中重现。使用了两种模型：一种是通过心脏穿刺抽取50%的血液且不进行回输，另一种是使动物经历60分钟的低血容量期（40毫米汞柱）并进行血液回输。在不同时间间隔处死动物，并采集胰腺组织进行形态学研究。光学显微镜观察未发现实验动物和对照动物之间存在差异。使用这些模型观察到的主要亚细胞改变是大量自噬泡的形成。从这些研究来看，休克时人胰腺中出现的改变在大鼠中可以重现，并且需要一种涉及去除动物超过50%血容量的休克模型才能对外分泌胰腺造成不可逆的细胞损伤。
