Zeng Chao-Xi, Qi Sui-Jian, Xin Rui-Pu, Yang Bo, Wang Yong-Hua
College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, 510640, China.
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, China.
Bioprocess Biosyst Eng. 2015 Nov;38(11):2053-61. doi: 10.1007/s00449-015-1445-0. Epub 2015 Jul 26.
In this study, enzymatic selective esterification of oleic acid with glycerol based on deep eutectic solvent acting as substrate and solvent was studied. As choline chloride (ChCl) or betaine can effectively change the chemical reaction characteristics of glycerol when they are mixed with a certain molar ratio of glycerol, several factors crucial to the lipase catalytic esterification of glycerol with oleic acid was investigated. Results showed that, betaine had more moderate effects than ChCl on the lipase, and water content had an important influence of the esterification and the enzyme selectivity. Significant changes of the glyceride compositions and enzyme selectivity were found in ChCl adding system compared with pure glycerol system; optimum accumulation of DAG especially 1,3-DAG because of the eutectic effect of ChCl was found in this system. Furthermore, in a model 1,3-DAG esterification synthesis system catalyzed by Novozym 435, high content (42.9 mol%) of the 1,3-DAG could be obtained in ChCl adding system within 1 h.
本研究以甘油为底物和溶剂，基于深共熔溶剂开展油酸与甘油的酶促选择性酯化反应研究。由于氯化胆碱（ChCl）或甜菜碱在与一定摩尔比的甘油混合时能有效改变甘油的化学反应特性，因此考察了几个对脂肪酶催化甘油与油酸酯化反应至关重要的因素。结果表明，甜菜碱对脂肪酶的影响比ChCl更为温和，水分含量对酯化反应和酶选择性有重要影响。与纯甘油体系相比，ChCl添加体系中甘油酯组成和酶选择性发生了显著变化；在该体系中，由于ChCl的共晶效应，发现二酰甘油尤其是1,3-二酰甘油有最佳积累。此外，在诺维信435催化的模型1,3-二酰甘油酯化合成体系中，ChCl添加体系在1小时内可获得高含量（42.9 mol%）的1,3-二酰甘油。
