Aarif Ovais, Aggarwal Anjali
Dairy Cattle Physiology Division, National Dairy Research Institute, Karnal, Haryana, India.
Dairy Cattle Physiology Division, National Dairy Research Institute, Karnal, Haryana, India.
Theriogenology. 2015 Oct 15;84(7):1197-205. doi: 10.1016/j.theriogenology.2015.06.019. Epub 2015 Jul 3.
The objective of the study was to observe the effect of evaporative cooling during late gestation on immunity around the transition period and the probable outcome on reproductive disorders in Murrah buffaloes. Sixteen pregnant dry Murrah buffaloes at 60 days prepartum were selected and divided into two groups of eight animals each. Group 1 buffaloes remained without the provision of cooling, whereas the second group of buffaloes was managed under fans and mist cooling during the dry period. After parturition, all the animals were managed under evaporative cooling. Dry matter intake was significantly (P < 0.05) higher in cooled relative to noncooled animals at -15, 0, and +20 days of parturition. Cortisol and prolactin levels were significantly (P < 0.05) higher in noncooled relative to cooled animals at -15 and 0 days of parturition. However, prolactin was significantly (P < 0.05) higher in cooled animals at +20 days. Messenger RNA expression of prolactin receptor gene (PRL-R) was upregulated and suppressor of cytokine signaling gene 1 (SOCS-1) was downregulated in cooled animals at -20, 0, and +20 days of parturition. Tumor necrosis factor α and interleukin 4 levels remained significantly (P < 0.05) higher in cooled animals at -20, 0, and +20 days of parturition. Interleukin 6 was significantly (P < 0.05) lower in cooled animals at -20 and 0 days. Interferon γ levels were significantly higher at -20 and +20 days of parturition in cooled relative to noncooled animals. The reproductive disorders such as retention of placenta, metritis, and endometritis occurred at the rate of 37.25%, 25%, and 12.25% in the noncooled group, whereas only retention of placenta was observed in the cooled (12.5%) group.
本研究的目的是观察妊娠后期蒸发冷却对围产期过渡期免疫力的影响以及对摩拉水牛繁殖障碍可能产生的结果。选择16头产前60天的怀孕干奶期摩拉水牛，分为两组，每组8头。第1组水牛不提供冷却措施，而第2组水牛在干奶期在风扇和喷雾冷却条件下饲养。分娩后，所有动物均在蒸发冷却条件下饲养。在分娩后-15、0和+20天时，冷却组动物的干物质摄入量显著（P<0.05）高于未冷却组。在分娩后-15和0天时，未冷却组动物的皮质醇和催乳素水平显著（P<0.05）高于冷却组。然而，在分娩后+20天时，冷却组动物的催乳素水平显著（P<0.05）更高。在分娩后-20、0和+20天时，冷却组动物催乳素受体基因（PRL-R）的信使核糖核酸表达上调，细胞因子信号传导抑制基因1（SOCS-1）下调。在分娩后-20、0和+20天时，冷却组动物的肿瘤坏死因子α和白细胞介素4水平仍显著（P<0.05）更高。在分娩后-20和0天时，冷却组动物的白细胞介素6显著（P<0.05）更低。在分娩后-20和+20天时，冷却组动物的干扰素γ水平相对于未冷却组显著更高。未冷却组中胎盘滞留、子宫炎和子宫内膜炎等繁殖障碍的发生率分别为37.25%、25%和12.25%，而冷却组（12.5%）仅观察到胎盘滞留。
