Zhang Qian-Qian, Li Fu-Gui, Qin Bo, Chen Jie, Jiang Xia-Yun, Zou Shu-Ming
Key Laboratory of Freshwater Aquatic Genetic Resources, Shanghai Ocean University, Huchenghuan Road 999, Shanghai 201306, China.
Key Laboratory of Freshwater Aquatic Genetic Resources, Shanghai Ocean University, Huchenghuan Road 999, Shanghai 201306, China.
Gene. 2015 Dec 1;573(2):225-32. doi: 10.1016/j.gene.2015.07.070. Epub 2015 Jul 23.
Fgfr1 is a fibroblast growth factor receptor involved in regulating cell growth, proliferation, differentiation and migration. Here, we report the isolation and characterization of duplicated fgfr1 genes in blunt snout bream (Megalobrama amblycephala). Blunt snout bream fgfr1a and -1b cDNAs were found to share a relatively high sequence identity of 82%. During embryogenesis, both fgfr1a and -1b mRNAs were highly detected at zygotes but gradually decreased and then constantly expressed after 16hpf, besides a strong expression for the fgfr1b mRNA at 12hpf. Whole-mount in situ hybridization demonstrated that fgfr1a mRNA was transcribed at the eyes, mid-hindbrain boundary (MHB), brain, posterior somites and tailbud at 16hpf, while the fgfr1b mRNA was only detected at the eyes and posterior somites at the same period. At 28hpf embryos, both fgfr1a and -1b mRNAs were expressed in the eyes, brain, pharyngeal arches and tailbud, and in the eyes, brain, pharyngeal arches and notochord at 55hpf. In adult fish, fgfr1a mRNA was strongly expressed in the gill, gonad, brain and midgut, but examined relatively low in the skin and kidney. In contrast, the fgfr1b mRNA was highly detected in the brain and liver and quite low in the skin, gill and kidney. During starvation, both fgfr1a and -1b mRNAs were significantly up-regulated in the intestine and liver, but down-regulated in the brain. Moreover, duplicated fgfr1 mRNAs were differentially inhibited in tissues with exogenous recombinant hGH. Our results suggest that two fgfr1 genes play important roles in regulating growth and development in blunt snout bream.
Fgfr1是一种成纤维细胞生长因子受体，参与调节细胞生长、增殖、分化和迁移。在此，我们报告了团头鲂（Megalobrama amblycephala）中重复的fgfr1基因的分离与鉴定。发现团头鲂fgfr1a和-1b cDNA具有82%的相对较高序列同一性。在胚胎发育过程中，fgfr1a和-1b mRNA在受精卵中均有高表达，但在16hpf后逐渐下降，然后持续表达，此外，fgfr1b mRNA在12hpf时有强表达。整体原位杂交显示，fgfr1a mRNA在16hpf时在眼睛、中后脑边界（MHB）、脑、后体节和尾芽处转录，而同期fgfr1b mRNA仅在眼睛和后体节处检测到。在28hpf胚胎中，fgfr1a和-1b mRNA均在眼睛、脑、咽弓和尾芽中表达，在55hpf时在眼睛、脑、咽弓和脊索中表达。在成年鱼中，fgfr1a mRNA在鳃、性腺、脑和中肠中强烈表达，但在皮肤和肾脏中的检测水平相对较低。相比之下，fgfr1b mRNA在脑中高度表达，在肝脏中表达较高，而在皮肤、鳃和肾脏中表达很低。在饥饿期间，fgfr1a和-1b mRNA在肠道和肝脏中均显著上调，但在脑中下调。此外，重复的fgfr1 mRNA在外源重组hGH处理的组织中受到不同程度的抑制。我们的结果表明，两个fgfr1基因在团头鲂的生长和发育调节中起重要作用。
