一种用于检测副溶血性弧菌的耦合垂直流可视化的交叉引物扩增测定法。
A cross-priming amplification assay coupled with vertical flow visualization for detection of Vibrio parahaemolyticus.
作者信息
Xu Deshun, Wu Xiaofang, Han Jiankang, Chen Liping, Ji Lei, Yan Wei, Shen Yuehua
机构信息
Huzhou Center for Disease Control and Prevention, Huzhou 313000, China.
Huzhou Center for Disease Control and Prevention, Huzhou 313000, China.
出版信息
Mol Cell Probes. 2015 Dec;29(6):527-530. doi: 10.1016/j.mcp.2015.07.007. Epub 2015 Jul 26.
Vibrio parahaemolyticus is a marine seafood-borne pathogen that causes gastrointestinal disorders in humans. In this study, we developed a cross-priming amplification (CPA) assay coupled with vertical flow (VF) visualization for rapid and sensitive detection of V. parahaemolyticus. This assay correctly detected all target strains (n = 13) and none of the non-target strains (n = 27). Small concentrations of V. parahaemolyticus (1.8 CFU/mL for pure cultures and 18 CFU/g for reconstituted samples) were detected within 1 h. CPA-VF can be applied at a large scale and can be used to detect V. parahaemolyticus strains rapidly in seafood and environmental samples, being especially useful in the field.
副溶血性弧菌是一种通过海产品传播的海洋病原体，可导致人类胃肠道疾病。在本研究中，我们开发了一种结合垂直流（VF）可视化的交叉引物扩增（CPA）检测方法，用于快速、灵敏地检测副溶血性弧菌。该检测方法正确检测出了所有目标菌株（n = 13），且未检测出任何非目标菌株（n = 27）。在1小时内检测到了低浓度的副溶血性弧菌（纯培养物中为1.8 CFU/mL，复溶样品中为18 CFU/g）。CPA-VF可大规模应用，可用于快速检测海产品和环境样品中的副溶血性弧菌菌株，在现场尤其有用。
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