School of Pharmacy, Fudan University, 826 Zhangheng Road, Shanghai 201203, China.
College of Fisheries and Life Science, 999 Hucheng Ring Road, Shanghai Ocean University, Shanghai 201306, China.
Food Chem. 2016 Jan 1;190:64-70. doi: 10.1016/j.foodchem.2015.05.059. Epub 2015 May 14.
A graphene-cobalt microsphere (CoMS) hybrid paste electrode was developed for the determination of carbohydrates in honey and milk in combination with capillary electrophoresis (CE). The performance of the electrodes was demonstrated by detecting mannitol, sucrose, lactose, glucose, and fructose after CE separation. The five analytes were well separated within 9 min in a 40 cm long capillary at a separation voltage of 12 kV. The electrodes exhibited pronounced electrocatalytic activity, lower detection potentials, enhanced signal-to-noise characteristics, and higher reproducibility. The relation between peak current and analyte concentration was linear over about three orders of magnitude. The proposed method had been employed to determine lactose in bovine milk and glucose and fructose in honey with satisfactory results. Because only electroactive substances in the samples could be detected on the paste electrode, the electropherograms of both food samples were simplified to some extent.
一种石墨烯-钴微球（CoMS）混合糊电极被开发出来，用于结合毛细管电泳（CE）测定蜂蜜和牛奶中的碳水化合物。通过 CE 分离后检测甘露醇、蔗糖、乳糖、葡萄糖和果糖，证明了电极的性能。在 40 厘米长的毛细管中，在 12 kV 的分离电压下，五种分析物在 9 分钟内得到了很好的分离。电极表现出明显的电催化活性、更低的检测电位、增强的信噪比特性和更高的重现性。在大约三个数量级的范围内，峰电流与分析物浓度呈线性关系。该方法已用于测定牛奶中的乳糖以及蜂蜜中的葡萄糖和果糖，结果令人满意。由于糊电极只能检测到样品中的电化学活性物质，因此两种食品样品的电泳图谱在一定程度上得到了简化。
