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大孔树脂对酱油中呈味肽的纯化。

Macroporous resin purification of peptides with umami taste from soy sauce.

机构信息

College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510650, China.

College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510650, China; Guangdong Food Green Processing and Nutrition Regulation Technologies Research Center, Guangzhou 510650, China.

出版信息

Food Chem. 2016 Jan 1;190:338-344. doi: 10.1016/j.foodchem.2015.05.105. Epub 2015 May 27.

Abstract

In this study, the performance and separation characteristics of four macroporous resins for purifying umami peptides from soy sauce were examined. Results showed that the resins could separate the peptides of soy sauce, and the particle diffusion kinetics model was suitable for describing the whole exothermic (ΔH < 43 kJ/mol) adsorption process on the SP-825 and HP-20 resins, while the pseudo-second-order kinetics model accurately described the XAD-16 and HP-2 MGL resins. Furthermore, the adsorption processes of the peptides followed the Freundlich model. The XAD-16 resin was the most effective resin for the enrichment of peptides due to its high adsorption and total desorption capacities. Interestingly, the umami peptides were enriched in the deionized water fraction. This study provides new insights into exploring performance and separation characteristics of macroporous resins on soy sauce, and indicated that peptide may be the contributor to the umami taste in Chinese soy sauce.

摘要

本研究考察了四种大孔树脂对酱油中鲜味肽的分离性能。结果表明,树脂可以分离酱油中的肽,且粒子扩散动力学模型适用于描述 SP-825 和 HP-20 树脂上的整个放热(ΔH < 43 kJ/mol)吸附过程,而准二级动力学模型准确地描述了 XAD-16 和 HP-2 MGL 树脂。此外,肽的吸附过程遵循弗雷德里希模型。由于 XAD-16 树脂具有较高的吸附和总解吸能力,因此它是富集肽的最有效树脂。有趣的是,鲜味肽富集在去离子水馏分中。本研究为探索大孔树脂对酱油的性能和分离特性提供了新的见解,并表明肽可能是中国酱油鲜味的贡献者。

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