Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School of Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan.
FUJI OIL CO., Ltd., Research and Development Division, 4-3, Kinunodai, Tsukuba, Ibaraki 300-2497, Japan.
Food Chem. 2016 Jan 1;190:345-350. doi: 10.1016/j.foodchem.2015.05.053. Epub 2015 May 28.
The aim of this study was to establish, through a standard addition method, a convenient quantification assay for dipeptides (GY, YG, SY, YS, and IY) in soybean hydrolysate using 2,4,6-trinitrobenzene sulfonate (TNBS) derivatization-aided LC-TOF-MS. Soybean hydrolysate samples (25.0 mg mL(-1)) spiked with target standards were subjected to TNBS derivatization. Under the optimal LC-MS conditions, five target dipeptides derivatized with TNBS were successfully detected. Examination of the standard addition curves, with a correlation coefficient of r(2) > 0.979, provided a reliable quantification of the target dipeptides, GY, YG, SY, YS, and IY, in soybean hydrolysate to be 424 ± 20, 184 ± 9, 2188 ± 199, 327 ± 16, and 2211 ± 133 μg g(-1) of hydrolysate, respectively. The proposed LC-MS assay is a reliable and convenient assay method, with no interference from matrix effects in hydrolysate, and with no requirement for the use of an isotope labeled internal standard.
本研究旨在通过 2,4,6-三硝基苯磺酸（TNBS）衍生化辅助 LC-TOF-MS 建立一种方便的大豆水解物中二肽（GY、YG、SY、YS 和 IY）定量分析方法。将含目标标准品的大豆水解物样品（25.0 mg mL(-1))进行 TNBS 衍生化。在最佳 LC-MS 条件下，成功检测到 5 种 TNBS 衍生化的目标二肽。标准加入曲线的考察，相关系数 r(2)＞0.979，为大豆水解物中目标二肽 GY、YG、SY、YS 和 IY 的可靠定量提供了依据，其含量分别为 424±20、184±9、2188±199、327±16 和 2211±133 μg g(-1)的水解物。所提出的 LC-MS 分析方法是一种可靠、方便的分析方法，水解物中无基质效应干扰，无需使用同位素标记的内标。
