Zuo Yi, Yuan Wanzhe, Sun Jiguo
College of Animal Medicine, Agriculture University of Hebei, Baoding 071001, China.
Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding 071001, China.
Genes (Basel). 2015 Jul 23;6(3):672-84. doi: 10.3390/genes6030672.
Porcine reproductive and respiratory syndrome virus (PRRSV) is the causal agent of a serious disease of swine. Here, we report the genome sequence of PRRSV strain HB-XL isolated from a pig farm with a clinical outbreak of porcine reproductive and respiratory syndrome. The genome is 15,323 bp long and has nine open reading frames (GenBank: KP162169). Comparative and phylogenetic analysis showed that HB-XL belongs to the highly pathogenic PRRSV (HP-PRRSV) subfamily in the family PRRSV. The viral nonstructural protein 2 (Nsp2) of the HB-XL strain contained 30 discontinuous amino acid (AA) deletions relative to that of the Nsp2 of the VR2332 strain. The AA substitutions R13 and R151 suggested high virulence of the HB-XL strain. The unique mutations in glycoprotein 5 (GP5) and Nsp2 revealed that HB-XL might be a novel variant PRRSV strain recombined with vaccine strains. However, the low morbidity and mortality in the pig herd from which HB-XL was isolated indicate that the virulence of the virus was weak, so it has potential as a future vaccine strain.
猪繁殖与呼吸综合征病毒（PRRSV）是猪的一种严重疾病的病原体。在此，我们报告从一个发生猪繁殖与呼吸综合征临床疫情的猪场分离出的PRRSV毒株HB-XL的基因组序列。该基因组长度为15323 bp，有9个开放阅读框（GenBank：KP162169）。比较和系统发育分析表明，HB-XL属于PRRSV科中的高致病性PRRSV（HP-PRRSV）亚家族。与VR2332毒株的Nsp2相比，HB-XL毒株的病毒非结构蛋白2（Nsp2）含有30个不连续的氨基酸（AA）缺失。氨基酸替代R13和R151表明HB-XL毒株具有高毒力。糖蛋白5（GP5）和Nsp2中的独特突变表明，HB-XL可能是一种与疫苗毒株重组的新型变异PRRSV毒株。然而，分离出HB-XL的猪群中发病率和死亡率较低，表明该病毒的毒力较弱，因此它有潜力成为未来的疫苗毒株。
