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一种用于检测碱基切除修复酶活性的荧光G-四链体探针。

A fluorescent G-quadruplex probe for the assay of base excision repair enzyme activity.

作者信息

Lee Chang Yeol, Park Ki Soo, Park Hyun Gyu

机构信息

Department of Chemical and Biomolecular Engineering (BK21 + Program), Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.

出版信息

Chem Commun (Camb). 2015 Sep 18;51(72):13744-7. doi: 10.1039/c5cc05010c. Epub 2015 Jul 27.

DOI:10.1039/c5cc05010c
PMID:26214499
Abstract

A sensitive and selective assay of uracil DNA glycosylase (UDG) activity is developed by utilizing a G-quadruplex probe incorporating 2-aminopurine (2-AP), based on a novel design that excision reaction promoted by UDG triggers the formation of G-quadruplex structure with significant fluorescence enhancement of 2-AP within the probe.

摘要

通过利用一种掺入2-氨基嘌呤（2-AP）的G-四链体探针，开发了一种灵敏且选择性的尿嘧啶DNA糖基化酶（UDG）活性检测方法，该方法基于一种新颖的设计，即UDG促进的切除反应触发G-四链体结构的形成，同时探针内的2-AP荧光显著增强。

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