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[栽培蒙古黄芪根际土壤水提取物对其种子萌发及生理特性的影响]

[Effect of Water Extracts from Rhizosphere Soil of Cultivated Astragalus membranaceus var. mongholicus on It's Seed Germination and Physiological Characteristics].

作者信息

Lang Duo-yong, Fu Xue-yan, Rong Jia-wang, Zhang Xin-hui

出版信息

Zhong Yao Cai. 2015 Jan;38(1):11-3.

PMID:26214865
Abstract

OBJECTIVE

To explore the relationship between continuous cropping obstacle and autotoxicity of Astragalus membranaceus var. mongholicus.

METHODS

Distilled water(CK), water extracts of rhizosphere soil(50, 100, 200 and 400 mg/mL) were applied to test their effect on early growth and physiological characteristics of Astragalus membranaceus var. mongholicus.

RESULTS

The water extracts from rhizospher soil of cultivated Astragalus membranaceus var. mongholicus significantly increased seedling emergence rate, root length and vigor index of Astragalus membranaceus var. mongholicus seedling when at the concentration of 100 mg/mL or below, however,there was no significant effect at 200 mg/mL or higher. The water extracts from rhizosphere soil of cultivated Astragalus membranaceus var. mongholicus significantly reduced the SOD activity in Astragalus membranaceus var. mongholicus seedling at 400 mg/mL and POD activity at 200 mg/mL and 400 mg/mL,while significantly increased the MDA content.

CONCLUSION

Water extracts from Astragalus membranaceus var. mongholicus rhizosphere soil significantly affected Astragalus membranaceus var. mongholicus germination and seedling growth in a concentration-dependent manner, generally, low concentrations increased the SOD and POD activity which improved seed germination and seedling growth, while high concentrations caused cell membrane damage of the seedling.

摘要

目的

探讨蒙古黄芪连作障碍与自毒作用的关系。

方法

分别用蒸馏水(CK)、根际土壤水提液(50、100、200和400mg/mL)处理,测定其对蒙古黄芪幼苗早期生长和生理特性的影响。

结果

栽培蒙古黄芪根际土壤水提液在浓度为100mg/mL及以下时,显著提高了蒙古黄芪幼苗的出苗率、根长和活力指数,而在200mg/mL及以上时无显著影响。栽培蒙古黄芪根际土壤水提液在400mg/mL时显著降低了蒙古黄芪幼苗的超氧化物歧化酶(SOD)活性,在200mg/mL和400mg/mL时显著降低了过氧化物酶(POD)活性,同时显著增加了丙二醛(MDA)含量。

结论

蒙古黄芪根际土壤水提液对蒙古黄芪种子萌发和幼苗生长有显著的浓度依赖性影响,一般低浓度提高SOD和POD活性,促进种子萌发和幼苗生长,高浓度则导致幼苗细胞膜损伤。

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