Hong Yuan-lin, Jin Yu-qing, Yao Yi-xin, Lin Mao-yi, Wei Bo-ping, Jiang Wei-dong, Lu Guang-hua
Zhong Yao Cai. 2015 Jan;38(1):36-40.
To quantity the amount of tetramethylpyrazine in Szechwan Lovage Rhizome (Chuanxiong, the rhizome of Ligusticum chuanxiong Hort., CX) and Cnidium Rhizome(Japanese Chuanxiong, the rhizome of Cnidium officinale Makino, JCX) for quality assessment.
An HPLC-DAD-MS technique was employed to detect tetramethylpyrazine in 27 CX and 10 JCX samples. Tetramethylpyrazine was separated on a Waters Symmetry C,, column (250 mm x 4. 6 mm, 5 µm). The mobile phase was methanol-acetonitrile-water(27: 1: 72) at a flow rate of 1. 0 mL/min. The column temperature was 35 °C. DAD detection wavelength was 280 nm, while electrospray ionization detector was set at positive mode to collect MS spectrum.
In the total of 37 herb samples, 11 samples were found to contain tetramethylpyrazine with the mean amount of 2. 19 µg/g(n = 11). 6 of 27 CX samples and 5 of 10 JCX sample were found the existence of tetramethylpyrazine with the amount of 0. 60 - 11. 75 µg and 0. 61 - 3. 05 µg/g,respectively. The correlation was not found between tetramethylpyrazine and the cultivation area, morphological character, processing or storage method for CX and JCX samples. It was possible that tetramethylpyrazine resulted from the microbes in soil.
The developed method is accurate to quantify tetramethylpyrazine in CX and JCX herbs. Both the two herbs indeed contain tetramethylpyrazine, but it is not suitable to be a chemical marker to assess the quality of CX and JCX owing to low content.
测定川芎（川穹，川芎Ligusticum chuanxiong Hort.的根茎，CX）和日本川芎（日本川穹，Cnidium officinale Makino的根茎，JCX）中川芎嗪的含量，以进行质量评估。
采用高效液相色谱 - 二极管阵列检测器 - 质谱联用（HPLC - DAD - MS）技术检测27份CX样品和10份JCX样品中的川芎嗪。川芎嗪在Waters Symmetry C18柱（250 mm×4.6 mm，5 µm）上分离。流动相为甲醇 - 乙腈 - 水（27:1:72），流速为1.0 mL/min。柱温为35℃。二极管阵列检测器检测波长为280 nm，电喷雾电离检测器设置为正离子模式采集质谱图。
在总共37份药材样品中，发现11份样品含有川芎嗪，平均含量为2.19 µg/g（n = 11）。27份CX样品中的6份和10份JCX样品中的5份存在川芎嗪，含量分别为0.60 - 11.75 µg和0.61 - 3.05 µg/g。未发现CX和JCX样品中川芎嗪与种植面积、形态特征、加工或储存方法之间存在相关性。川芎嗪可能是由土壤中的微生物产生的。
所建立的方法可准确测定CX和JCX药材中川芎嗪的含量。两种药材确实都含有川芎嗪，但由于含量较低，不适宜作为评估CX和JCX质量的化学指标。
