Cutró A C, Disalvo E A
Laboratorio de Biointerfases y Sistemas Biomiméticos, Laboratorios Centrales, Centro de Investigaciones y Transferencia Santiago del Estero, Universidad Nacional de Santiago del Estero, CP 4206 Santiago del Estero, Argentina.
J Phys Chem B. 2015 Aug 6;119(31):10060-5. doi: 10.1021/acs.jpcb.5b05590. Epub 2015 Jul 28.
We study the binding of phenylalanine (Phe) with dipalmitoylphosphocholine (DPPC) vesicles in gel (25 °C) and in liquid crystalline states (50 °C) and in gel large unilamellar vesicles (LUVs) subjected to osmotic dehydration with merocyanine (MC 540) as a fluorescent surface membrane marker. Phe does not produce significant changes in MC 540 monomer concentration in DPPC LUVs at 50 °C. In contrast, it significantly decreases the monomer adsorption in defects present in DPPC LUVs at 25 °C. When DPPC LUVs were subjected to hypertonic stress, dehydration caused more defects, and in this case phenylalanine is also able to block such defects.
我们研究了苯丙氨酸（Phe）与二棕榈酰磷脂酰胆碱（DPPC）囊泡在凝胶态（25°C）、液晶态（50°C）以及用部花青（MC 540）作为荧光表面膜标记物进行渗透脱水处理的凝胶态大单层囊泡（LUVs）中的结合情况。在50°C时，Phe不会使DPPC LUVs中MC 540单体浓度产生显著变化。相比之下，在25°C时，它会显著降低DPPC LUVs中缺陷处的单体吸附。当DPPC LUVs受到高渗应激时，脱水会导致更多缺陷，在这种情况下苯丙氨酸也能够阻断此类缺陷。
