Takeshima Yasuhiro, Miyake Hitoshi, Nakagawa Ichiro, Motoyama Yasushi, Park Young-Su, Nakase Hiroyuki
Department of Neurosurgery, Nara Medical University School of Medicine, Kashihara, Japan.
Department of Neurosurgery, Nara Medical University School of Medicine, Kashihara, Japan.
J Stroke Cerebrovasc Dis. 2015 Oct;24(10):2200-6. doi: 10.1016/j.jstrokecerebrovasdis.2015.04.048. Epub 2015 Jul 26.
Cerebral venous ischemia (CVI) is a rare but potentially significant complication of neurosurgical procedures. However, it is still unclear how cerebral venous occlusion (VO) affects regional cerebral blood flow (rCBF) dynamics. To elucidate its pathophysiology in detail, we examined the real-time perfusion dynamics during adjacent vein occlusions using laser speckle contrast imaging (LSCI) in a rat 2-vein occlusion model.
Two cortical veins were occluded photochemically using rose Bengal dye in 6 male Wistar rats; rCBF was measured in real time with an LSCI before and after VO. Regions of interest were defined between the 2 veins (A) and on the opposite side of the first occluded vein (B) on semi-quantitative pseudocolor images for off-line analysis. Histopathologic evaluation was performed 3 days after the procedure to assess the extent of infarction.
LSCI revealed a stepwise reduction in CBF, with a sudden decrease just after the first vein occlusion (20%) and a further decrease after the second (30%). Significant differences were observed between rCBF dynamics within regions of interest A and B (P = .0004). All rats exhibited infarcts in the superficial cerebral cortex histopathologically.
This is the first report of LSCI specifically applied to the study of CVI. The extensive real-time measurement with high temporal and spatial resolution revealed the stepwise reduction in rCBF during sequential VO and the ensuing infarcts.
脑静脉缺血（CVI）是神经外科手术中一种罕见但潜在严重的并发症。然而，脑静脉闭塞（VO）如何影响局部脑血流（rCBF）动力学仍不清楚。为了详细阐明其病理生理学，我们在大鼠双静脉闭塞模型中使用激光散斑对比成像（LSCI）检查了相邻静脉闭塞期间的实时灌注动力学。
在6只雄性Wistar大鼠中，使用孟加拉玫瑰红染料光化学闭塞两条皮质静脉；在VO前后用LSCI实时测量rCBF。在半定量伪彩色图像上，在两条静脉之间（A）和第一条闭塞静脉的对侧（B）定义感兴趣区域，用于离线分析。术后3天进行组织病理学评估，以评估梗死范围。
LSCI显示CBF呈逐步降低，在第一条静脉闭塞后立即突然下降（约20%），第二条静脉闭塞后进一步下降（约30%）。在感兴趣区域A和B内的rCBF动力学之间观察到显著差异（P = 0.0004）。所有大鼠在组织病理学上均表现为大脑皮质浅层梗死。
这是首次将LSCI专门应用于CVI研究的报告。具有高时间和空间分辨率的广泛实时测量揭示了连续VO期间rCBF的逐步降低以及随后的梗死。
