特应性皮炎患者接受窄谱中波紫外线光疗后循环中的CD4(+) CD25(高表达) FoxP3(+) 调节性T细胞
Circulating CD4(+) CD25(high) FoxP3(+) T-regulatory cells in patients with atopic dermatitis after narrowband-ultraviolet B phototherapy.
作者信息
El Samahy May H, Attia Enas A S, Saad Abeer A, Mahmoud Eman Y
机构信息
Department of Dermatology, Venereology, and Andrology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
Department of Clinical Pathology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
出版信息
Int J Dermatol. 2015 Oct;54(10):e424-9. doi: 10.1111/ijd.12801. Epub 2015 Jul 28.
BACKGROUND
Previous studies showed controversial results regarding CD4(+) CD25(high) FoxP3(+) T-regulatory cells (Tregs) in atopic dermatitis (AD) and effect of therapy.
METHODS
Circulating CD4(+) CD25(high) FoxP3(+) Tregs were assessed by flow cytometry in 20 controls and 20 patients with AD at baseline and after narrowband ultraviolet B with assessment of disease severity.
RESULTS
Patients showed higher pretreatment T-effector cells (Teffs) (%) and lower pretreatment Tregs FoxP3 expression% than controls (P = 0.003 and 0.01, respectively). Mild AD showed a lower Tregs/Teffs ratio compared to controls (P = 0.013), while moderate group showed higher Teffs%, and lower Tregs FoxP3 expression% and Tregs/Teffs compared to controls (P = 0.016, 0.007, and 0.009 respectively). The severe group had higher Tregs% and Teffs%, yet with a lower Tregs FoxP3 expression% compared to controls (P < 0.001, P = 0.043, P = 0.044, respectively). There was significant reduction of severity after narrowband ultraviolet B (P = 0.007), with overall significant elevation of Tregs FoxP3 expression% in patients (P = 0.004). All patients' post-treatment laboratory findings were statistically matched to each other and to controls whatever their previous severity or therapeutic response. The improvement of severity score correlated with the change in both Tregs% and Tregs/Teffs.
CONCLUSIONS
Significant reduction in AD disease severity is correlated with the change in Tregs% and Tregs/Teffs.
背景
先前的研究显示，关于特应性皮炎（AD）中CD4(+) CD25(高) FoxP3(+) 调节性T细胞（Tregs）及治疗效果的结果存在争议。
方法
通过流式细胞术对20名对照者和20名AD患者在基线时以及接受窄谱中波紫外线B治疗后循环中的CD4(+) CD25(高) FoxP3(+) Tregs进行评估，并评估疾病严重程度。
结果
与对照者相比，患者治疗前的效应T细胞（Teffs）百分比更高，治疗前Tregs的FoxP3表达百分比更低（分别为P = 0.003和0.01）。轻度AD患者的Tregs/Teffs比值低于对照者（P = 0.013），而中度AD组与对照者相比，Teffs百分比更高，Tregs的FoxP3表达百分比及Tregs/Teffs更低（分别为P = 0.016、0.007和0.009）。重度AD组的Tregs百分比和Teffs百分比均高于对照者，但Tregs的FoxP3表达百分比低于对照者（分别为P < 0.001、P = 0.043、P = 0.044）。窄谱中波紫外线B治疗后疾病严重程度显著降低（P = 0.007），患者的Tregs FoxP3表达百分比总体显著升高（P = 0.004）。无论患者先前的严重程度或治疗反应如何，所有患者治疗后的实验室检查结果在统计学上相互匹配且与对照者匹配。严重程度评分的改善与Tregs百分比和Tregs/Teffs的变化相关。
结论
AD疾病严重程度的显著降低与Tregs百分比和Tregs/Teffs的变化相关。
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