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使用两种人乳脂肪球膜蛋白抗体（HMFG-1 和 HMFG-2）检测多形性腺瘤中的 MUC-1 表达。

MUC-1 expression in pleomorphic adenomas using two human milk fat globule protein membrane antibodies (HMFG-1 and HMFG-2).

机构信息

Facultad de Odontología, Universidad Nacional Autónoma de México, México, D.F, México.

出版信息

J Appl Oral Sci. 2015 May-Jun;23(3):265-71. doi: 10.1590/1678-775720140481.

DOI:10.1590/1678-775720140481
PMID:26221920
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4510660/
Abstract

UNLABELLED

Pleomorphic adenoma (PA) is the most common salivary gland tumor and its microscopic features and histogenesis are a matter of debate. Human milk fat globule protein membrane (HMFG) monoclonal antibodies (MoAbs) comprise a set of antibodies against the mucin 1 (MUC-1) protein detected in several salivary gland tumors.

OBJECTIVE

The aim of this study was to assess the immunoexpression of the PA neoplastic cells to MUC-1 protein using HMFG-1 and HMFG-2 MoAbs, contrasting these results with those from normal salivary gland tissue.

MATERIAL AND METHODS

Immunohistochemical detection of MUC-1 protein using HMFG-1 and HMFG-2 MoAbs was made in 5 mm thick, paraffin embedded slides, and the avidin-biotin method was used.

RESULTS

Positivity to HMFG-1 and HMFG-2 MoAbs was found in ductal, squamous metaplastic and neoplastic myoepithelial cells, keratin pearls and intraductal mucous material. Two kinds of myoepithelial cells were identified: classic myoepithelial cells around ducts were negative to both MoAbs, and modified myoepithelial cells were positive to both MoAbs. This last cellular group of the analyzed tumors showed similar MUC-1 immunoexpression to ductal epithelial cells using both HMFG antibodies. Intraductal mucous secretion was also HMFG-1 and HMFG-2 positive.

CONCLUSIONS

Our results showed there are two kinds of myoepithelial cells in PA. The first cellular group is represented by the different kinds of neoplastic myoepithelial cells and is HMFG-positive. The second one is HMFG-negative and represented by the neoplastic myoepithelial cells located around the ducts.

摘要

未加标签

多形性腺瘤（PA）是最常见的唾液腺肿瘤，其微观特征和组织发生一直存在争议。人乳脂肪球膜（HMFG）单克隆抗体（MoAbs）由一组针对几种唾液腺肿瘤中检测到的黏蛋白 1（MUC-1）蛋白的抗体组成。

目的

本研究旨在使用 HMFG-1 和 HMFG-2 MoAbs 评估 PA 肿瘤细胞对 MUC-1 蛋白的免疫表达，将这些结果与正常唾液腺组织的结果进行对比。

材料与方法

使用 HMFG-1 和 HMFG-2 MoAbs 对石蜡包埋的 5 毫米厚切片进行 MUC-1 蛋白的免疫组织化学检测，使用亲和素-生物素法。

结果

HMFG-1 和 HMFG-2 MoAbs 在导管、鳞状化生和肿瘤性肌上皮细胞、角蛋白珠和导管内黏液物质中呈阳性。鉴定出两种肌上皮细胞：围绕导管的经典肌上皮细胞对两种 MoAbs 均为阴性，而改良的肌上皮细胞对两种 MoAbs 均为阳性。在分析的肿瘤中，这种最后一种细胞群使用两种 HMFG 抗体对导管上皮细胞表现出相似的 MUC-1 免疫表达。导管内黏液分泌也呈 HMFG-1 和 HMFG-2 阳性。

结论

我们的结果表明 PA 中有两种肌上皮细胞。第一种细胞群由不同类型的肿瘤性肌上皮细胞组成，呈 HMFG 阳性。第二种细胞群呈 HMFG 阴性，由位于导管周围的肿瘤性肌上皮细胞组成。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f54/4510660/e91785f83f3b/1678-7757-jaos-23-3-0265-gf08.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f54/4510660/114b691f58f7/1678-7757-jaos-23-3-0265-gf01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f54/4510660/fe4ac0452656/1678-7757-jaos-23-3-0265-gf02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f54/4510660/d9b018c77b1f/1678-7757-jaos-23-3-0265-gf03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f54/4510660/242541d4d254/1678-7757-jaos-23-3-0265-gf04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f54/4510660/21dfe1bfac75/1678-7757-jaos-23-3-0265-gf05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f54/4510660/8113a8ed8ea8/1678-7757-jaos-23-3-0265-gf06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f54/4510660/99aa89bdb264/1678-7757-jaos-23-3-0265-gf07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f54/4510660/e91785f83f3b/1678-7757-jaos-23-3-0265-gf08.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f54/4510660/114b691f58f7/1678-7757-jaos-23-3-0265-gf01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f54/4510660/fe4ac0452656/1678-7757-jaos-23-3-0265-gf02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f54/4510660/d9b018c77b1f/1678-7757-jaos-23-3-0265-gf03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f54/4510660/242541d4d254/1678-7757-jaos-23-3-0265-gf04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f54/4510660/21dfe1bfac75/1678-7757-jaos-23-3-0265-gf05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f54/4510660/8113a8ed8ea8/1678-7757-jaos-23-3-0265-gf06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f54/4510660/99aa89bdb264/1678-7757-jaos-23-3-0265-gf07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9f54/4510660/e91785f83f3b/1678-7757-jaos-23-3-0265-gf08.jpg

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本文引用的文献

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Histopathology. 2011 Feb;58(3):377-82. doi: 10.1111/j.1365-2559.2011.03758.x. Epub 2011 Feb 16.
2
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APMIS. 2008 Feb;116(2):93-8. doi: 10.1111/j.1600-0463.2008.00771.x.
3
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J Clin Pathol. 2004 Aug;57(8):813-21. doi: 10.1136/jcp.2003.014043.
4
Parietal cell carcinoma of gastric cardia: immunophenotype and ultrastructure.贲门胃壁细胞癌：免疫表型与超微结构
Ultrastruct Pathol. 2003 Mar-Apr;27(2):87-94. doi: 10.1080/01913120309923.
5
Salivary gland tumours in a Mexican sample. A retrospective study.墨西哥样本中的唾液腺肿瘤。一项回顾性研究。
Med Oral. 2002 Nov-Dec;7(5):324-30.
6
Polymorphous low-grade adenocarcinoma of the salivary glands with transformation to high-grade carcinoma.涎腺多形性低度恶性腺癌伴向高级别癌转化
Histopathology. 2002 Sep;41(3):250-9. doi: 10.1046/j.1365-2559.2002.01439.x.
7
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J Investig Dermatol Symp Proc. 2001 Nov;6(1):49-53. doi: 10.1046/j.0022-202x.2001.00005.x.
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Anticancer Res. 2000 Jul-Aug;20(4):2321-4.
9
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