Joris L, Quinton P M
Division of Biomedical Sciences, University of California, Riverside 92521-0121.
Pflugers Arch. 1989 Oct;415(1):118-20. doi: 10.1007/BF00373149.
We demonstrate that electrogenic glucose coupled Na+ absorption accounts for about 20% of the residual, amiloride and Cl- insensitive, Na+ transport in isolated (equine) trachea. Either glucose removal from the mucosal side or addition of 10(-4) M phloridzin, a known pharmacological inhibitor of Na-glucose cotransport in small intestine and kidney proximal tubule, results in depolarization (lumen referenced to bath) of the transepithelial potential difference (Vt) and in a significant decrease of the equivalent short circuit current Ieq by 4 mV and 4 microA/cm2, respectively, without affecting the transepithelial resistance (Rt). Neither glucose removal nor the application of phloridzin on the basolateral side have a significant or consistent effect on the bioelectric parameters.
我们证明，在分离的（马）气管中，电生性葡萄糖偶联的Na⁺吸收占残余的、对氨氯吡咪和Cl⁻不敏感的Na⁺转运的约20%。从黏膜侧去除葡萄糖或添加10⁻⁴ M根皮苷（一种已知的小肠和肾近端小管中Na⁺-葡萄糖共转运的药理学抑制剂），会导致跨上皮电位差（Vt）去极化（管腔相对于浴液），等效短路电流Ieq分别显著降低4 mV和4 μA/cm²，而不影响跨上皮电阻（Rt）。从基底外侧去除葡萄糖或应用根皮苷对生物电参数均无显著或一致的影响。
