School of Resources and Environmental Sciences, University of Jinan, Jinan 250022, PR China.
School of Resources and Environmental Sciences, University of Jinan, Jinan 250022, PR China.
Biosens Bioelectron. 2015 Dec 15;74:786-91. doi: 10.1016/j.bios.2015.07.049. Epub 2015 Jul 22.
An ultrasensitive sandwich-type electrochemical immunosensor was designed for the quantitative detection of alpha fetoprotein (AFP). The β-cyclodextrins functionalized graphene sheets (CD-GS) were used as the sensing matrix for immobilizing adamantine-1-carboxylic acid functionalized primary anti-AFP (ADA-Ab1) and enhanced the electron transfer. PdNi alloy nanoparticles decorated N-doped graphene nanoribbons (PdNi/N-GNRs) were used as labels of secondary anti-AFP (Ab2), and PdNi alloy nanoparticles (PdNi NPs) exhibited high catalytic performance towards the reduction of H2O2. Meanwhile, with good dispersion, large specific surface area and good catalytic performance, N-doped graphene nanoribbons (N-GNRs) significantly amplified the electrochemical signal. Under the optimal conditions, the electrochemical immunosensor exhibited a wide linear range of 0.0001-16 ng/mL with a low detection limit of 0.03 pg/mL. Additionally, the proposed immunosensor showed high specificity, good reproducibility and long-term stability, which have promising application in bioassay analysis.
一种超灵敏的夹心型电化学免疫传感器被设计用于甲胎蛋白（AFP）的定量检测。β-环糊精功能化石墨烯片（CD-GS）被用作固定金刚烷-1-羧酸功能化的一级抗 AFP（ADA-Ab1）和增强电子转移的传感基质。PdNi 合金纳米粒子修饰的 N 掺杂石墨烯纳米带（PdNi/N-GNRs）被用作二级抗 AFP（Ab2）的标记物，并且 PdNi 合金纳米粒子（PdNi NPs）对 H2O2 的还原表现出高催化性能。同时，N 掺杂石墨烯纳米带（N-GNRs）具有良好的分散性、大的比表面积和良好的催化性能，显著放大了电化学信号。在最佳条件下，电化学免疫传感器表现出 0.0001-16ng/mL 的宽线性范围和 0.03pg/mL 的低检测限。此外，所提出的免疫传感器表现出高特异性、良好的重现性和长期稳定性，在生物分析中有很好的应用前景。
