Liang Aihui, Li Chongning, Li Dan, Luo Yanghe, Wen Guiqing, Jiang Zhiliang
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University, Ministry of Education.
Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin University of Technology, Guilin.
Int J Nanomedicine. 2017 Dec 12;12:8725-8734. doi: 10.2147/IJN.S149536. eCollection 2017.
The nanogold reaction between HAuCl and citrate is very slow, and the catalyst graphene oxide nanoribbon (GONR) enhanced the nanoreaction greatly to produce gold nanoparticles (AuNPs) that exhibited strong surface plasmon resonance (SPR) absorption (Abs) at 550 nm and resonance Rayleigh scattering (RRS) at 550 nm. Upon addition of the peptide of human chorionic gonadotropin (hCG), the peptide could adsorb on the GONR surface, which inhibited the catalysis. When hCG was added, peptides were separated from the GONR surface due to the formation of stable peptide-hCG complex, which led to the activation of GONR catalytic effect. With the increase in hCG concentration, the RRS and Abs signal enhanced linearly. The enhanced RRS value showed a good linear relationship with hCG concentration in the range of 0.2-20 ng/mL, with a detection limit of 70 pg/mL. Accordingly, two new GONR catalytic RRS/Abs methods were established for detecting hCG in serum samples.
氯金酸（HAuCl）与柠檬酸盐之间的纳米金反应非常缓慢，而催化剂氧化石墨烯纳米带（GONR）极大地增强了该纳米反应，从而生成金纳米颗粒（AuNPs），这些金纳米颗粒在550 nm处表现出强烈的表面等离子体共振（SPR）吸收（Abs）以及在550 nm处的共振瑞利散射（RRS）。加入人绒毛膜促性腺激素（hCG）肽后，该肽可吸附在GONR表面，从而抑制催化作用。当加入hCG时，由于形成稳定的肽 - hCG复合物，肽从GONR表面分离，这导致GONR催化作用的激活。随着hCG浓度的增加，RRS和Abs信号呈线性增强。在0.2 - 20 ng/mL范围内，增强的RRS值与hCG浓度呈现良好的线性关系，检测限为70 pg/mL。据此，建立了两种新的GONR催化RRS/Abs方法用于检测血清样品中的hCG。
