Rehbein Grit, Schmidt Bernd, Fleischhacker Michael
Abteilung Pneumologie, Klinik für Innere Medizin 1, Martin-Luther-Universität Halle-Wittenberg, Ernst-Grube-Straße 40, 06120 Halle/Saale, Germany.
Clin Chim Acta. 2015 Oct 23;450:78-82. doi: 10.1016/j.cca.2015.07.027. Epub 2015 Jul 30.
The detection of tumor-associated microRNA expression in bronchial lavage (BL) samples of lung cancer patients could improve the non-invasive tumor diagnostic.
The profile of extracellular microRNAs in bronchial lavage was evaluated using three pools for lung cancer group (malignant) and three pools for non-cancerous group (benign) of 10 patients each. To confirm the results for the selected microRNAs in a qRT-PCR the BL of 30 different lung cancer and non-cancerous patients was used. We examined total-RNA from cell-free supernatant of BL samples. For normalization we added exogenous cel-miR 39.
Using microRNA arrays we found a panel of eight microRNAs (hsa-miR 19b-1, 1285, 1289, 1303, 217, 29a-5p, 548-3p, 650) that were differentially expressed between the lung cancer and the non-cancerous group. Further investigation by qPCR revealed five microRNAs (U6 snRNA, hsa-miR 1285, 1303, 29a-5p, 650) that were significantly up-regulated in patients with lung cancer.
In bronchial lavage samples the five microRNAs identified in this study have a diagnostic potential for use as noninvasive biomarkers in lung cancer.
检测肺癌患者支气管灌洗（BL）样本中肿瘤相关微小RNA的表达，可改善非侵入性肿瘤诊断。
使用三个样本池评估支气管灌洗中细胞外微小RNA的特征，其中肺癌组（恶性）和非癌组（良性）各有10名患者的样本池。为通过定量逆转录聚合酶链反应（qRT-PCR）确认所选微小RNA的结果，使用了30名不同肺癌和非癌患者的支气管灌洗样本。我们检测了支气管灌洗样本无细胞上清液中的总RNA。为进行标准化，我们添加了外源性cel-miR 39。
使用微小RNA阵列，我们发现一组八个微小RNA（hsa-miR 19b-1、1285、1289、1303、217、29a-5p、548-3p、650）在肺癌组和非癌组之间差异表达。通过qPCR进一步研究发现五个微小RNA（U6小核RNA、hsa-miR 1285、1303、29a-5p、650）在肺癌患者中显著上调。
在支气管灌洗样本中，本研究鉴定出的五个微小RNA具有作为肺癌非侵入性生物标志物的诊断潜力。
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