肾脏替代治疗类型（血液透析与腹膜透析）不影响肾移植受者的细胞因子基因表达或临床参数。

Type of Renal Replacement Therapy (Hemodialysis versus Peritoneal Dialysis) Does Not Affect Cytokine Gene Expression or Clinical Parameters of Renal Transplant Candidates.

作者信息

Kamińska Dorota, Kościelska-Kasprzak Katarzyna, Chudoba Paweł, Mazanowska Oktawia, Banasik Mirosław, Żabinska Marcelina, Boratyńska Maria, Lepiesza Agnieszka, Korta Krzysztof, Gomółkiewicz Agnieszka, Dzięgiel Piotr, Klinger Marian

机构信息

Department of Nephrology and Transplantation Medicine, Wroclaw Medical University, Borowska 213, 50-556 Wrocław, Poland.

Department of Vascular, General and Transplant Surgery, Wroclaw Medical University, Borowska 213, 50-556 Wrocław, Poland.

出版信息

Biomed Res Int. 2015;2015:797490. doi: 10.1155/2015/797490. Epub 2015 Jul 8.

DOI:10.1155/2015/797490

PMID:26236736

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4510116/

Abstract

UNLABELLED

Patients with renal failure suffer from immune disturbances, caused by uremic toxins and influenced by dialysis treatment. The aim of the present study was to reveal whether type of dialysis modality (hemodialysis, HD, versus peritoneal dialysis, PD) differentially affects the immunocompetence, particularly the expression of genes involved in the immune response.

MATERIAL

87 renal transplant candidates (66 HD, 21 PD) were included in the study.

METHODS

The peripheral blood RNA samples were obtained with the PAXgene Blood system just before transplantation. The gene expression of CASP3, FAS, TP53, FOXP3, IFNG, IL2, IL6, IL8, IL10, IL17, IL18, LCN2, TGFB1, and TNF was assessed with real-time PCR on custom-designed low density arrays (TaqMan). Gene expression data were analyzed in relation to pretransplant clinical parameters.

RESULTS

The mean expression of examined genes showed no significant differences between PD and HD with the exception of FAS, expression of which was 30% higher in PD patients compared to the HD group. There was nonsignificantly higher expression of proinflammatory cytokines in the PD group. The clinical inflammatory parameters (CRP, albumin, cholesterol, and hemoglobin levels) did not differ between the groups.

CONCLUSION

Type of renal replacement therapy exerts no differential effect on cytokine gene expression or inflammatory clinical parameters.

摘要

未标注

肾衰竭患者存在免疫紊乱，这是由尿毒症毒素引起并受透析治疗影响的。本研究的目的是揭示透析方式（血液透析，HD，与腹膜透析，PD）是否对免疫能力有不同影响，特别是参与免疫反应的基因表达。

材料

87名肾移植候选者（66名HD，21名PD）被纳入研究。

方法

在移植前，使用PAXgene血液系统获取外周血RNA样本。通过在定制设计的低密度阵列（TaqMan）上进行实时PCR，评估CASP3、FAS、TP53、FOXP3、IFNG、IL2、IL6、IL8、IL10、IL17、IL18、LCN2、TGFB1和TNF的基因表达。基因表达数据与移植前临床参数相关进行分析。