Morita Tetsuro, Morishima Kagayaki, Miwa Misako, Kumakura Naoki, Kudo Satomi, Ichida Kensuke, Mitsuboshi Toru, Takeuchi Yutaka, Yoshizaki Goro
Central Research Laboratory, Nippon Suisan Kaisha, Ltd., 1-32-3 Nanakuni, Hachioji-shi, Tokyo, 192-0991, Japan,
Mar Biotechnol (NY). 2015 Oct;17(5):644-54. doi: 10.1007/s10126-015-9657-5. Epub 2015 Aug 4.
Production of xenogeneic gametes from large-bodied, commercially important marine species in closely related smaller surrogates with short generation times may enable rapid domestication of the targeted species. In this study, we aimed to produce gametes of Japanese yellowtail (Seriola quinqueradiata) using jack mackerel (Trachurus japonicus) as a surrogate with a smaller body size and shorter maturation period. Donor spermatogonia were collected from the testes of yellowtail males and transferred into the peritoneal cavity of 10- and 12-day-old jack mackerel larvae. Twenty days later, 59.5% of the recipients survived of which 88.2% had donor-derived germ cells in their gonads. One year later, genomic DNA templates were prepared from the semen of 96 male recipients and subjected to polymerase chain reaction (PCR) analyses using primers specific for the yellowtail vasa sequence, resulting in the detection of positive signals in semen from two recipients. The milt collected from the recipients was used for fertilization with yellowtail eggs. Of eight hatchlings obtained from the crosses, two were confirmed to be derived from donor yellowtail by DNA markers, although the others were gynogenetic diploids. These findings indicate that it is possible to produce donor-derived sperm in xenogeneic recipients with a smaller body size and shorter generation time by transplanting spermatogonia. Thus, the xenogeneic transplantation of spermatogonia might be a potential tool to produce gametes of large-bodied, commercially important fish, although the efficiency of the method requires further improvement. This is the first report demonstrating that donor-derived sperm could be produced in xenogeneic recipient via spermatogonial transplantation in carangid fishes.
利用体型较小、世代时间短的近缘替代物种来生产大型、具有商业重要性的海洋物种的异种配子，可能会使目标物种快速驯化。在本研究中，我们旨在以体型较小且成熟期较短的竹荚鱼作为替代物种，来生产日本黄尾鰤的配子。从黄尾鰤雄性的睾丸中收集供体精原细胞，并将其转移到10日龄和12日龄的竹荚鱼幼体的腹腔中。20天后，59.5%的受体存活下来，其中88.2%的受体性腺中有供体来源的生殖细胞。一年后，从96只雄性受体的精液中制备基因组DNA模板，并使用针对黄尾鰤vasa序列的特异性引物进行聚合酶链反应（PCR）分析，结果在两只受体的精液中检测到阳性信号。从受体收集的精液用于使黄尾鰤卵受精。在杂交获得的8只幼体中，通过DNA标记确认有2只是来自供体黄尾鰤，尽管其他幼体是雌核发育二倍体。这些发现表明，通过移植精原细胞，有可能在体型较小且世代时间短的异种受体中产生供体来源的精子。因此，精原细胞的异种移植可能是生产大型、具有商业重要性鱼类配子的一种潜在工具，尽管该方法的效率需要进一步提高。这是第一份证明通过鲹科鱼类精原细胞移植可在异种受体中产生供体来源精子的报告。
