Chan S P, Onyekaba C O, Harty J T, Plagemann P G
Department of Microbiology, Medical School, University of Minnesota, Minneapolis 55455.
Virus Res. 1989 Dec;14(4):317-26. doi: 10.1016/0168-1702(89)90024-5.
In order to assess whether the spleen is the major site of replication of lactate dehydrogenase-elevating virus (LDV) in mice during the acute phase of infection, LDV replication in the spleen was measured by electron microscopy and fluorescent antibody staining of tissue sections and northern hybridization of total spleen RNA with an LDV-specific cDNA probe, and the effect of splenectomy on LDV replication was determined. LDV RNA and antigens and infected cells, presumably macrophages, were present in the spleen in high concentrations 18-25 h post infection, but then rapidly disappeared to undetectable levels during the next 1-2 days. Thus, LDV clearly replicates in the spleen during the initial phase of infection, but LDV replication in the spleen is transient due to the cytocidal nature of LDV replication and destruction of all permissive macrophages in the spleen. Furthermore, spleen macrophages do not seem to represent the major source of LDV released into the circulation, since LDV viremia as well as anti-LDV antibody production were the same in splenectomized and control animals for at least 28 days postinfection.
为了评估在感染急性期乳酸脱氢酶升高病毒（LDV）在小鼠体内复制的主要部位是否为脾脏，通过电子显微镜、组织切片的荧光抗体染色以及用LDV特异性cDNA探针进行脾脏总RNA的Northern杂交来检测脾脏中LDV的复制情况，并确定脾切除对LDV复制的影响。感染后18 - 25小时，脾脏中存在高浓度的LDV RNA、抗原以及被感染的细胞（推测为巨噬细胞），但在接下来的1 - 2天内迅速消失至检测不到的水平。因此，LDV在感染初期确实在脾脏中复制，但由于LDV复制的杀细胞特性以及脾脏中所有允许其感染的巨噬细胞被破坏，脾脏中LDV的复制是短暂的。此外，脾脏巨噬细胞似乎并非循环中释放的LDV的主要来源，因为在感染后至少28天内，脾切除动物和对照动物的LDV病毒血症以及抗LDV抗体产生情况相同。
