经导管动脉化疗栓塞术对肝细胞癌患者细胞免疫功能及调节性T细胞的影响
Effect of transcatheter arterial chemoembolization on cellular immune function and regulatory T cells in patients with hepatocellular carcinoma.
作者信息
Liao Juan, Xiao Jiangwei, Zhou Yunfeng, Liu Zilin, Wang Chunhui
机构信息
Department of Gastroenterology, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China.
Department of General Surgery, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China.
出版信息
Mol Med Rep. 2015 Oct;12(4):6065-71. doi: 10.3892/mmr.2015.4171. Epub 2015 Aug 3.
The present study aimed to investigate changes in cellular immune function and regulatory T cells (Tregs) in patients with hepatocellular carcinoma (HCC) prior to and following transcatheter arterial chemoembolization (TACE) and their clinical significance. The proportion of CD4+ and CD8+ T cells and Tregs in the peripheral blood between healthy volunteers and patients with HCC were detected by flow cytometry prior to (1 day) and one month following TACE. The level of interleukin (IL)‑35 in the peripheral blood was examined by ELISA. In the peripheral blood of patients with HCC, the proportion of CD4+ T cells in the total T lymphocytes was significantly lower compared with that of healthy volunteers (26.71 ± 5.57, vs. 34.74 ± 2.86%; P<0.05) and the ratio of CD4+/CD8+ T lymphocytes in patients with HCC was lower compared with that of healthy adults prior to TACE (1.03 ± 0.14, vs. 1.68 ± 0.16, P<0.05). The ratio markedly increased following TACE treatment (30.52 ± 4.19, vs. 1.29 ± 0.14). The percentage of CD4+CD25+ Treg cells in the total CD4+ T cells isolated from the patients with HCC was markedly higher compared with that of healthy adults prior to TACE (11.12 ± 3.58%, vs. 4.98 ± 1.45%, P<0.05) and it was significantly decreased following TACE (7.58±2.65%; P<0.05). No statistically significant difference in the expression of IL‑35 was detected prior to or following TACE in patients with HCC and healthy adults (369.66 ± 95.53, 352.28 ± 107.50 and 316.24 ± 89.21 pg/ml, respectively). The level of AFP, an oncofetal protein of ~72 kDa, which is produced by normal gastrointestinal cells, yolk sac cells and fetal hepatocytes immediately following birth, was increased in patients with HCC (1674 ± 1649 ng/ml) and was significantly decreased following TACE (827 ± 981 ng/ml). Treg cells changed in positive correlation with the change of AFP, with a correlation coefficient of 0.401. TACE markedly improved the immune function of patients with HCC.
本研究旨在探讨肝细胞癌（HCC）患者经肝动脉化疗栓塞术（TACE）前后细胞免疫功能及调节性T细胞（Tregs）的变化及其临床意义。采用流式细胞术检测健康志愿者和HCC患者在TACE术前（1天）及术后1个月外周血中CD4⁺和CD8⁺T细胞及Tregs的比例。采用酶联免疫吸附测定（ELISA）法检测外周血白细胞介素（IL）-35水平。HCC患者外周血中，总T淋巴细胞中CD4⁺T细胞的比例显著低于健康志愿者（26.71±5.57% vs. 34.74±2.86%；P<0.05），且TACE术前HCC患者CD4⁺/CD8⁺T淋巴细胞比值低于健康成年人（1.03±0.14 vs. 1.68±0.16，P<0.05）。TACE治疗后该比值显著升高（30.52±4.19 vs. 1.29±0.14）。HCC患者分离出的总CD4⁺T细胞中CD4⁺CD25⁺Treg细胞的百分比显著高于TACE术前的健康成年人（11.12±3.58% vs. 4.98±1.45%，P<0.05），且TACE术后显著降低（7.58±2.65%；P<0.05）。HCC患者和健康成年人在TACE术前或术后IL-35表达水平差异均无统计学意义（分别为369.66±95.53、352.28±107.50和316.24±89.21 pg/ml）。甲胎蛋白（AFP）是一种约72 kDa的癌胚蛋白，由正常胃肠细胞、卵黄囊细胞和出生后即刻的胎儿肝细胞产生，HCC患者AFP水平升高（1674±1649 ng/ml），TACE术后显著降低（827±981 ng/ml）。Treg细胞变化与AFP变化呈正相关，相关系数为0.401。TACE显著改善了HCC患者的免疫功能。
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