Poo M, Lam J W, Orida N, Chao A W
Biophys J. 1979 Apr;26(1):1-21. doi: 10.1016/S0006-3495(79)85231-5.
Electrophoretic and diffusional movements of concanavalin A (Con A) receptors and acetylcholine (ACh) receptors in the plane of the plasma membrane of mononucleate, spherical Xenopus myoblasts were studied by microfluorimetry and iontophoresis. We found that (a) a uniform electric field of 10 V/cm applied along the cell surface produces a partial accumulation of both types of receptors toward the cathodal pole of the cell within 30 min: (b) post-field relaxation of the culture results in the complete recovery of the uniform distribution of the Con A receptors within 10 min; and (c) in contrast to the Con A receptor in general, accumulation of ACh receptors by the electric field results in the formation of stable, localized receptor aggregates. Theoretical analyses were carried out for the distribution of charged membrane receptors at equilibrium between electrophoresis and diffusion, and for the rate of back diffusion after the removal of the field. These analyses indicated that, at 22 degrees C, the average electrophoretic mobility of the electrophoretically mobile population of the Con A receptors is about 1.9 X 10(-3) micron/s per V/cm, while their average diffusion coefficient is 5.1 X 10(-9) cm2/s.
通过显微荧光测定法和离子电泳法，研究了单核球形非洲爪蟾成肌细胞质膜平面上伴刀豆球蛋白A（Con A）受体和乙酰胆碱（ACh）受体的电泳和扩散运动。我们发现：（a）沿细胞表面施加10 V/cm的均匀电场，会在30分钟内使两种类型的受体都向细胞的阴极极部分积累；（b）电场作用后培养物的弛豫导致Con A受体在10分钟内完全恢复均匀分布；（c）与一般的Con A受体不同，电场使ACh受体积累会导致形成稳定的局部受体聚集体。对带电膜受体在电泳和扩散平衡时的分布以及去除电场后的反向扩散速率进行了理论分析。这些分析表明，在22℃时，Con A受体电泳可移动群体的平均电泳迁移率约为每V/cm 1.9×10⁻³微米/秒，而其平均扩散系数为5.1×10⁻⁹平方厘米/秒。
