Okamoto Masaaki, Imai Susumu, Miyanohara Mayu, Saito Wataru, Momoi Yasuko, Nomura Yoshiaki, Ikawa Tomoko, Ogawa Takumi, Miyabe-Nishiwaki Takako, Kaneko Akihisa, Watanabe Akino, Watanabe Shohei, Hayashi Misato, Tomonaga Masaki, Hanada Nobuhiro
Department of Translational Research.
Department of Operative Dentistry.
Microbiol Immunol. 2015 Sep;59(9):526-32. doi: 10.1111/1348-0421.12290.
Three strains TKU9, TKU49 and TKU50(T) , were isolated from the oral cavities of chimpanzees (Pan troglodytes). The isolates were all gram-positive, facultative anaerobic cocci that lacked catalase activity. Analysis of partial 16S rRNA gene sequences showed that the most closely related species was Streptococcus infantis (96.7%). The next most closely related species to the isolates were S. rubneri, S. mitis, S. peroris and S. australis (96.6 to 96.4%). Based on the rpoB and gyrB gene sequences, TKU50(T) was clustered with other member of the mitis group. Enzyme activity and sugar fermentation patterns differentiated this novel bacterium from other members of the mitis group streptococci. The DNA G + C content of strain TKU50(T) was 46.7 mol%, which is the highest reported value for members of the mitis group (40-46 mol%). On the basis of the phenotypic characterization, partial 16S rRNA gene and sequences data for two housekeeping gene (gyrB and rpoB), we propose a novel taxa, S. panodentis for TKU 50(T) (type strain = CM 30579(T) = DSM 29921(T) ), for these newly described isolates.
从黑猩猩（Pan troglodytes）口腔中分离出三株菌株TKU9、TKU49和TKU50(T)。这些分离株均为革兰氏阳性兼性厌氧球菌，缺乏过氧化氢酶活性。对部分16S rRNA基因序列的分析表明，与之亲缘关系最近的物种是婴儿链球菌（96.7%）。与这些分离株亲缘关系次近的物种是鲁氏链球菌、缓症链球菌、牙周链球菌和澳大利亚链球菌（96.6%至96.4%）。基于rpoB和gyrB基因序列，TKU50(T)与轻链球菌组的其他成员聚在一起。酶活性和糖发酵模式将这种新细菌与轻链球菌组的其他成员区分开来。菌株TKU50(T)的DNA G + C含量为46.7 mol%，这是轻链球菌组报道的最高值（40 - 46 mol%）。基于表型特征、部分16S rRNA基因以及两个管家基因（gyrB和rpoB）的序列数据，我们为这些新描述的分离株提出一个新分类单元，即口腔泛生链球菌TKU 50(T)（模式菌株 = CM 30579(T) = DSM 29921(T)）。
