用于观察阳离子脂质体细胞运输的新型胆固醇基荧光脂质的合成与验证。
Synthesis and validation of novel cholesterol-based fluorescent lipids designed to observe the cellular trafficking of cationic liposomes.
作者信息
Kim Bieong-Kil, Seu Young-Bae, Choi Jong-Soo, Park Jong-Won, Doh Kyung-Oh
机构信息
School of Life Sciences and Biotechnology, Kyungpook National University, Daegu 702-701, Republic of Korea; Department of Physiology, College of Medicine, Yeungnam University, Daegu 705-717, Republic of Korea.
School of Life Sciences and Biotechnology, Kyungpook National University, Daegu 702-701, Republic of Korea.
出版信息
Bioorg Med Chem Lett. 2015 Sep 15;25(18):3893-6. doi: 10.1016/j.bmcl.2015.07.051. Epub 2015 Jul 23.
Cholesterol-based fluorescent lipids with ether linker were synthesized using NBD (Chol-E-NBD) or Rhodamine B (Chol-E-Rh), and the usefulnesses as fluorescent probes for tracing cholesterol-based liposomes were validated. The fluorescent intensities of liposomes containing these modified lipids were measured and observed under a microscope. Neither compound interfered with the expression of GFP plasmid, and live cell images were obtained without interferences. Changes in the fluorescent intensity of liposomes containing Chol-E-NBD were followed by flow cytometry for up to 24h. These fluorescent lipids could be useful probes for trafficking of cationic liposome-mediated gene delivery.
使用NBD（胆固醇-醚-NBD）或罗丹明B（胆固醇-醚-罗丹明）合成了具有醚连接基的基于胆固醇的荧光脂质，并验证了其作为追踪基于胆固醇的脂质体的荧光探针的实用性。测量了含有这些修饰脂质的脂质体的荧光强度，并在显微镜下进行观察。两种化合物均未干扰GFP质粒的表达，并且获得了无干扰的活细胞图像。通过流式细胞术跟踪含有胆固醇-醚-NBD的脂质体荧光强度的变化长达24小时。这些荧光脂质可能是用于阳离子脂质体介导的基因递送的有用探针。
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