Mandal M, Banerjee S, Majumder G C
Indian Institute of Chemical Biology, Jadavpyur, Calcutta.
Biol Reprod. 1989 Dec;41(6):983-9. doi: 10.1095/biolreprod41.6.983.
Blood sera of humans, rats, goats, and buffalo have been shown to possess a forward motility-stimulating factor (FMSF) that markedly stimulated goat cauda epididymal sperm forward motility, as assayed by a microscopic method in the presence of epididymal plasma (1.2 mg protein/ml) that had sufficient anti-sticking activity to eliminate the possibility of cell-sticking artifacts in motility assays. The specific activity of FMSF was greatest in buffalo blood serum compared to the sera of the other species. Buffalo serum at a concentration as low as 8.5 mg protein/ml induced forward motility in nearly 45% of the cells. The buffalo serum FMSF was heat-stable, nondialyzable, and sensitive to the action of trypsin. Purified proteins--casein, serum albumin, ovalbumin, myoglobin, and beta-lactoglobulin--showed little or relatively low FMSF activity. FMSF is a glycoprotein, as it binds with high affinity to concanavalin A-agarose. A major portion of the serum protein (approx. 70%) did not bind to the affinity matrix, and this unretained serum protein fraction showed little FMSF activity. The FMSF activity of buffalo serum was confirmed by estimating sperm forward motility spectrophotometrically: an objective method of assessing sperm motility.
已证明人类、大鼠、山羊和水牛的血清中含有一种正向运动刺激因子（FMSF），该因子能显著刺激山羊附睾尾精子的正向运动。在附睾血浆（1.2毫克蛋白质/毫升）存在的情况下，通过显微镜方法进行测定，附睾血浆具有足够的抗黏附活性，可消除运动测定中细胞黏附假象的可能性。与其他物种的血清相比，FMSF在水牛血清中的比活性最高。浓度低至8.5毫克蛋白质/毫升的水牛血清可使近45%的细胞产生正向运动。水牛血清FMSF具有热稳定性、不可透析性，且对胰蛋白酶的作用敏感。纯化的蛋白质——酪蛋白、血清白蛋白、卵清蛋白、肌红蛋白和β-乳球蛋白——显示出很少或相对较低的FMSF活性。FMSF是一种糖蛋白，因为它与伴刀豆球蛋白A-琼脂糖具有高亲和力结合。大部分血清蛋白（约70%）不与亲和基质结合，且该未保留的血清蛋白部分显示出很少的FMSF活性。通过分光光度法估计精子正向运动来确认水牛血清的FMSF活性：这是一种评估精子运动的客观方法。
