Sun Yanfa, Liu Ranran, Zhao Guiping, Zheng Maiqing, Sun Yan, Yu Xiaoqiong, Li Peng, Wen Jie
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China College of Life Science, Longyan University, Longyan, Fujian 364012, People's Republic of China Fujian Provincial Key Laboratory of Preventive Veterinary Medicine and Biotechnology, Longyan University, Longyan, Fujian 364012, People's Republic of China.
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China State Key Laboratory of Animal Nutrition, Beijing 100193, People's Republic of China.
G3 (Bethesda). 2015 Aug 6;5(10):2037-41. doi: 10.1534/g3.115.020883.
Physical appearance traits, such as feather-crested head, comb size and type, beard, wattles size, and feathered feet, are used to distinguish between breeds of chicken and also may be associated with economic traits. In this study, a genome-wide linkage analysis was used to identify candidate regions and genes for physical appearance traits and to potentially provide further knowledge of the molecular mechanisms that underlie these traits. The linkage analysis was conducted with an F2 population derived from Beijing-You chickens and a commercial broiler line. Single-nucleotide polymorphisms were analyzed using the Illumina 60K Chicken SNP Beadchip. The data were used to map quantitative trait loci and genes for six physical appearance traits. A 10-cM/0.51-Mb region (0.0-10.0 cM/0.00-0.51 Mb) with 1% genome-wide significant level on LGE22C19W28_E50C23 linkage group (LGE22) for crest trait was identified, which is likely very closely linked to the HOXC8. A QTL with 5% chromosome-wide significant level for comb weight, which partly overlaps with a region identified in a previous study, was identified at 74 cM/25.55 Mb on chicken (Gallus gallus; GG) chromosome 3 (i.e., GGA3). For beard and wattles traits, an identical region 11 cM/2.23 Mb (0.0-11.0 cM/0.00-2.23 Mb) including WNT3 and GH genes on GGA27 was identified. Two QTL with 1% genome-wide significant level for feathered feet trait, one 9-cM/2.80-Mb (48.0-57.0/13.40-16.20 Mb) region on GGA13, and another 12-cM/1.45-Mb (41.0-53.0 cM/11.37-12.82 Mb) region on GGA15 were identified. These candidate regions and genes provide important genetic information for the physical appearance traits in chicken.
身体外观特征，如羽冠头、鸡冠大小和类型、胡须、肉垂大小以及有羽毛的脚，可用于区分鸡的品种，并且可能与经济性状相关。在本研究中，进行了全基因组连锁分析，以确定身体外观特征的候选区域和基因，并有可能进一步了解这些性状背后的分子机制。连锁分析是使用来自北京油鸡和一个商业肉鸡品系的F2群体进行的。使用Illumina 60K鸡SNP芯片分析单核苷酸多态性。这些数据用于绘制六个身体外观特征的数量性状位点和基因图谱。在LGE22C19W28_E50C23连锁群（LGE22）上，针对羽冠性状确定了一个全基因组显著水平为1%的10厘摩/0.51兆碱基区域（0.0 - 10.0厘摩/0.00 - 0.51兆碱基），该区域可能与HOXC8紧密连锁。在鸡（原鸡；GG）3号染色体（即GGA3）上74厘摩/25.55兆碱基处，确定了一个鸡冠重量全染色体显著水平为5%的数量性状位点，该位点部分重叠于先前研究中确定的一个区域。对于胡须和肉垂性状，在GGA27上确定了一个相同的区域，即11厘摩/2.23兆碱基（0.0 - 11.0厘摩/0.00 - 2.23兆碱基），该区域包含WNT3和GH基因。确定了两个全基因组显著水平为1%的有羽毛的脚性状的数量性状位点，一个在GGA13上的9厘摩/2.80兆碱基区域（48.0 - 57.0/13.40 - 16.20兆碱基），另一个在GGA15上的12厘摩/1.45兆碱基区域（41.0 - 53.0厘摩/11.37 - 12.82兆碱基）。这些候选区域和基因为鸡的身体外观性状提供了重要的遗传信息。
