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使用X射线相衬显微断层扫描技术对大卫两栖甲(鞘翅目,两栖甲科)的外部和内部结构进行研究

The external and internal structures of Amphizoa davidi Lucas (Coleoptera, Amphizoidae), using X-ray phase contrast microtomography.

作者信息

Li Dee, Zhang Kai, Li Xiaoyan, Zhu Peiping, Xu Caifeng, Wu Ziyu, Zhou Hongzhang

机构信息

Key Laboratory of the Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, 1 Beichen West Road, Chao Yang, 100101 Beijing, P. R. China; Email: unknown.

Beijing Synchrotron Radiation Facility, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, P. R. China; Email: unknown.

出版信息

Zootaxa. 2015 May 27;3963(3):335-68. doi: 10.11646/zootaxa.3963.3.3.

DOI:10.11646/zootaxa.3963.3.3
PMID:26249404
Abstract

The Chinese endemic water beetle Amphizoa davidi Lucas, is a rare and endangered species belonging to the monotypic family Amphizoidae (Coleoptera: Adephaga). A study of the external and internal structures of A. davidi is here presented, by using X-ray phase contrast tomography and light microscopy. Morphological details and three dimensional (3D) structures of this species are provided: skeletons, muscles, reproductive organs of male and female, nervous system, alimentary canal and pygidial gland. The reproductive organs of females are compared in two different developmental phases (ages): before copulation without mature ovaries and after copulation with mature ovaries. Such detailed 3D tomographic study based on micro-CT technology may promote our understanding of the detailed morphology in Amphizoidae and Coleoptera in general.

摘要

中国特有水生甲虫大卫两栖甲(Amphizoa davidi Lucas)是一种珍稀濒危物种,隶属于单型科两栖甲科(鞘翅目:肉食亚目)。本文通过X射线相衬断层扫描和光学显微镜,对大卫两栖甲的外部和内部结构进行了研究。提供了该物种的形态细节和三维(3D)结构:骨骼、肌肉、雌雄生殖器官、神经系统、消化道和臀腺。比较了处于两个不同发育阶段(年龄)的雌性生殖器官:交配前未成熟卵巢阶段和交配后成熟卵巢阶段。基于显微CT技术的如此详细的3D断层扫描研究,总体上可能会促进我们对两栖甲科和鞘翅目详细形态的理解。

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