Rosadas Carolina, Puccioni-Sohler Marzia
Cerebrospinal Fluid Laboratory, Hospital Universitário Clementino Fraga Filho, Universidade Federal do Rio de Janeiro, Rua Professor Rodolpho Paulo Rocco 255, 3°andar, Rio de Janeiro, 21941-913, Brazil.
Laboratório de Líquido Cefalorraquiano, Serviço de Patologia Clínica, Hospital Universitário Clementino Fraga Filho, Universidade Federal do Rio de Janeiro, Rua Professor Rodolpho Paulo Rocco 255, 3 ± andar, Rio de Janeiro, RJ, 21941-913, Brazil.
J Biomed Sci. 2015 Aug 8;22(1):66. doi: 10.1186/s12929-015-0170-y.
Different human retroviruses, such as Human Immunodeficiency Virus (HIV) and Human T-cell Lymphotropic Virus (HTLV), can cause neurologic infection. However, a definitive diagnosis may be hampered by several factors. Quantification of the viral or proviral load in cerebrospinal fluid (CSF) may be helpful in the diagnosis of nervous system disorders due to retroviral infection and may influence the treatment approach. The present work discusses retrovirus infection and neurologic impairment, as well as the usefulness of the determination of the HIV and HTLV proviral or viral load in cerebrospinal fluid in cases of neurologic disorder, in light of recent advances in this field. This study also discusses the different molecular techniques for quantifying the proviral load (real-time quantitative PCR, droplet digital PCR, and semi-nested real-time reverse transcription PCR) that are currently available.
不同的人类逆转录病毒，如人类免疫缺陷病毒（HIV）和人类嗜T细胞病毒（HTLV），可引起神经感染。然而，一些因素可能会妨碍做出明确的诊断。脑脊液（CSF）中病毒或前病毒载量的定量分析可能有助于诊断由逆转录病毒感染引起的神经系统疾病，并可能影响治疗方法。鉴于该领域的最新进展，本研究讨论了逆转录病毒感染与神经功能损害，以及在神经系统疾病病例中测定脑脊液中HIV和HTLV前病毒或病毒载量的作用。本研究还讨论了目前可用于定量前病毒载量的不同分子技术（实时定量PCR、数字液滴PCR和半巢式实时逆转录PCR）。
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