Hasanein P, Teimuri-Far M
School of Basic Sciences, Bu—Ali Sina University Department of Biology Hamedan Iran p.hasanein@basu.ac.ir.
School of Basic Sciences, Bu—Ali Sina University Department of Biology Hamedan Iran.
Cell Mol Biol (Noisy-le-grand). 2015 Aug 5;61(4):8-14.
Oxidative stress is among the mechanisms involved in renal injury. We aimed to investigate the protective effects of bioactive peptide carnosine on lead induced oxidative stress and nephrotoxicity in rats. Animals received an aqueous solution of lead acetate (500 mg Pb/L in the drinking water) and/or carnosine (10 mg/kg, i.g.) for eight weeks. Then rats were weighed and used for biochemical, histological and oxidant/antioxidant evaluations. Lead-induced oxidative stress in renal tissue was indicated by a significant increase in the renal contents of malondialdehyde (MDA) as well as decrease in the levels of reduced glutathione (GSH), total antioxidant capacity (TAC), catalase (CAT) and superoxide dismutase (SOD) (P's < 0.001). Carnosine treatment decreased MDA whereas it increased the contents of GSH, TAC, CAT and SOD in both lead and control groups. Carnosine prevented the increased kidney weight and lead-induced deleterious effects on serum creatinine, urea, uric acid, albumin and total protein in lead group. It also attenuated lead induced abnormal renal structure. The present study showed that carnosine protected against lead induced oxidative stress and renal injury in rat. Therefore, carnosine represents a potential therapeutic option against the deleterious effect of lead induced nephrotoxicity which deserves consideration and further examination.
氧化应激是参与肾损伤的机制之一。我们旨在研究生物活性肽肌肽对铅诱导的大鼠氧化应激和肾毒性的保护作用。动物接受醋酸铅水溶液（饮用水中含500 mg Pb/L）和/或肌肽（10 mg/kg，灌胃），持续8周。然后对大鼠称重，并用于生化、组织学和氧化/抗氧化评估。肾组织中丙二醛（MDA）含量显著增加，同时还原型谷胱甘肽（GSH）、总抗氧化能力（TAC）、过氧化氢酶（CAT）和超氧化物歧化酶（SOD）水平降低，表明铅诱导了肾组织的氧化应激（P值<0.001）。肌肽处理降低了MDA含量，同时增加了铅处理组和对照组中GSH、TAC、CAT和SOD的含量。肌肽可防止铅处理组肾脏重量增加以及铅对血清肌酐、尿素、尿酸、白蛋白和总蛋白的有害影响。它还减轻了铅诱导的肾脏结构异常。本研究表明，肌肽可保护大鼠免受铅诱导的氧化应激和肾损伤。因此, 肌肽是一种针对铅诱导肾毒性有害作用的潜在治疗选择，值得考虑和进一步研究。
