Pitter Demar R G, Brown Adrienne S, Baker James D, Wilson James N
Department of Chemistry, University of Miami, 1301 Memorial Drive, Coral Gables, FL 33146, USA.
Org Biomol Chem. 2015 Sep 28;13(36):9477-84. doi: 10.1039/c5ob01428j. Epub 2015 Aug 10.
Several new DNA-targeting probes that exhibit binding-induced 'turn on' fluorescence are presented. Two of the dyes, orange emissive 1, (E)-4-(4(-4-methylpiperazin-1-yl)phenyl)6-(4-(4-methylpi-perazin-1-yl)styryl)pyrimidin-2-ol), and red emissive 2, (E)-4-(4(-4-methyl-piperazin-1-yl)-phenyl)6-(4-(4-methylpiperazin-1-yl)styryl)-1,3-propanedionato-κO,κO']difluoroborane), are brightly fluorescent when bound to DNA, but are virtually non-fluorescent in aqueous solutions. Confocal fluorescence microscopy of live BT474, MCF7 and HEK293 cells demonstrates that both probes are cell permeable and rapidly accumulated intracellularly into cell nuclei and the cytosol. Taking advantage of their environmental sensitivity, these two pools of fluorophores are readily resolved into separate channels, and thus, a single dye allows two-color imaging of the nuclear and cytosolic compartments.
本文介绍了几种新的靶向DNA的探针，这些探针具有结合诱导的“开启”荧光特性。其中两种染料，橙色发射的1（(E)-4-(4(-4-甲基哌嗪-1-基)phenyl)6-(4-(4-甲基哌嗪-1-基)苯乙烯基)嘧啶-2-醇）和红色发射的2（(E)-4-(4(-4-甲基哌嗪-1-基)phenyl)6-(4-(4-甲基哌嗪-1-基)苯乙烯基)-1,3-丙二酮基-κO,κO']二氟硼烷），与DNA结合时具有明亮的荧光，但在水溶液中几乎不发荧光。对活的BT474、MCF7和HEK293细胞进行共聚焦荧光显微镜观察表明，这两种探针都具有细胞渗透性，并能迅速在细胞内积累到细胞核和细胞质中。利用它们对环境的敏感性，这两类荧光团很容易被分辨到不同的通道中，因此，单一染料就能对细胞核和细胞质区域进行双色成像。
